Sequences from confirmation amplicon


Confirmation sequences for line 824G07 and hit At1g50650 are shown.
Line ID 824G07
Locus At1g50650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18764463
Primers used for PCR BD52 - 8474, show primer details
   
Sequence Name 30-K028349-022-824-G07C-8409
GenBank Accession LN499588 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K028349-022-824-G07C-8409
ACAACATCATCCATGCAAGAAAAATCATGAACACTCCCTAAATCTTTCTCTCTCTTCAAA
CTTATATAAACCCTTCATTAATTAGACATCTCAATCAATGATCTAAATAACTAAACTCAT
CATCACAAAGTCATCTCAATGATGAGCATTAAGCTGACATTGTGTGCCTTAATCTTCTTC
CTCTTAAATTCACTTCTTCATCATGTTCTCGGTCACGATAACCAACTTAACACAACCTCG
TCATGGCTTAAGAGCCACATCAAAGCAGCTACAACAACAAATTGGGGTAGACCAAAGCCA
CCGATGTGTAAACCATGGATTTGCAAACGGTCCGGACCATCAGAGGCTCGAATGAGATGT
TGTCGTAACCAATGCGTTGATGTCTTGTCCGATCCAAACCATTGTCGGTT
   
Sequence Name 30-K028349-022-824-G07C-BD52
GenBank Accession LN499589 [GenBank]
Sequencing primer gene specific primer, BD52: 5'-AACTCTTGAAACAGAACCGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K028349-022-824-G07C-BD52
AAAATTCCATTGGGTTTAACACATTCGGTTGGCTTTTTGGTCTACCCCAATTTGTCGTTG
TAGCCGCTTTGATGTGGCTCTTAAGCCATGACGAGGTTGTGTTAAGTTGGTTATCGTGAC
CGAGAACATGATGAAGAAGTGAATTTAAGAGGAAGAAGATTAAGGCACACAATGTCAGCT
TAATGCTCATCATTGAGATGACTTTGTGATGATGAGTTTAGTTATTTAGATCATTGATTG
AGATGTCTAATTAATGAAGGGTTTATATAAGTTTGAAGAGAGAGAAAGATTTAGGGAGTG
TTCATGATTTTCTTGCATGGATGATGTTGTCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT
Primers used for PCR 66wl - 8474, show primer details
   
Sequence Name 43-K118446-0022-824-G07C-66wl
GenBank Accession LN505001 [GenBank]
Sequencing primer gene specific primer, 66wl: 5'-AGTTATTTCGTCCTGTCTCGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118446-0022-824-G07C-66wl
AGCATACCATATTAATCACACTTCTGGTTTGTTTTGCTTTCTTTCTTCTTAATTTCTTCT
TGACCTGAAATTTCATTGCATACATACTCTTATATTCATGAGTTATCAGGGACTAACTTA
TACTTGTTTCTTTTCTTTAAACTGAAAGCACTAAGTGAAAACTTTTGTGGCCTTTTCTCA
TCGATTAAATATACAATAATTTCGAAAGAACCAACAAAATTTGCTATGCGATCCGCAACT
TGCTAATGATAACATAGCAAGATTAGGTCGAACTCCTCTCCGTACGCTTGTAAGGTTTGC
GCAATTGTGCTCAATGAGATCCGATTAGTCCGTCCATATCTTGATTAATATTTTTGTTAA
CTATAAAATTTTAATAAGACCAAGAATAACTCCATACGAATATGTGTTCGCATGCCATCA
TTTAGGACAGACCAAAGTGAAAAGAAAAAAAGAAATGTAATCCCAATATTTTCTTAACAA
CCTTTTGTAACCCCCAAAGAAAATAAAACTTAGCAAAATTCATGTGGGCTTTGTTAACCC
ACTTTTGTTCTTCCAACTACATTGCCATGTGATACCCTTTTCTTTTCTTTTCTTCACACT
ATTACTATAAATGTACTCCTCGCGGTCCAAACATAAATTAATTAGAAGATTAATAAATTT
TAAAGGGTCATATTAATGAATAATAAGATGAACGTAACAATGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CANAAATGTAG
   
Sequence Name 03-K118445-0022-824-G07C-8409
GenBank Accession LN487684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118445-0022-824-G07C-8409
TATTCATTATATGACCCTTTAAATTTATTAATCTTCTAATTAATTTATGTTTGGACCGCG
AGGAGTACATTTATAGTAATAGTGTGAAGAAAAGAAAAGAAAAGGGTATCACATGGCAAT
GTAGTTGGAAGAACAAAAGTGGGTTAACAAAGCCCACATGAATTTTGCTAAGTTTTATTT
TCTTTGGGGGTTACAAAAGGTTGTTAAGAAAATATTGGGATTACATTTCTTTTTTTCTTT
TCACTTTGGTCTGTCCTAAATGATGGCATGCGAACACATATTCGTATGGAGTTATTCTTG
GTCTTATTAAAATTTTATAGTTAACAAAAATATTAATCAAGATATGGACGGACTAATCGG
ATCTCATTGAGCACAATTGCGCAAACCTTACAAGCGTACGGAGAGGAGTTCGACCTAATC
TTGCTATGTTATCATTAGCAAGTTGCGGATCGCATAGCAAATTTTGTTGGTTCTTTCGAA
ATTATTGTATATTTAATCGATGAGAAAAGGCCACAAAAGTTTTCACTTAGTGCTTTCAGT
TTAAAGAAAAGAAACAAGTATAAGTTAGTCCCTGATAACTCATGAATATAAGAGTATGTA
TGCAATGAAATTTCAGGTCAAGAAGAAATTAAGAAGAAAGAAAGCAAAACAAACCAGAAG
TGTGATTAATATGGTATGCTATAATTGGGCTACAAGGCCCGTAATGTAAAAGGCTTGTTT
TCGAGACAG

Last Updated on 06.12.2018 16:11