Sequences from confirmation amplicon


Confirmation sequences for line 825F01 and hit At2g30070 are shown.
Line ID 825F01
Locus At2g30070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12837419
Primers used for PCR ST09 - 8474, show primer details
   
Sequence Name 53-K103548-0022-825-F01C-ST09
GenBank Accession LN508125 [GenBank]
Sequencing primer gene specific primer, ST09: 5'-GTGTTTGATACTTGCATACATGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103548-0022-825-F01C-ST09
ATATTCGCAAAGCTTCTACAAGGCGATACCCGGTAAATTCTTAACTGTAATCTGTATAGT
ACGCGAATAACTCGAGACTTTTGAATGGGATTAAGTCAGATAAGTCTGTTTGCAGAGCCT
GTGTTCTGGCCAGTGTTCATTGTGGCTACATTTGCAGCTGTGGTGGGAAGCCAAGCTGTC
ATATCCGCAACGTTTTCGATCATAAGTCAATGTTGTGCACTTGATTGCTTTCCTCGGGTC
AAGATCATTCACACTTCAAGCAAAATACAATATGTAGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 37-K103549-0022-825-F01C-8409
GenBank Accession LN502586 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103549-0022-825-F01C-8409
ATTTACATTGAAATATCTACATATTGTATTTTGCTTGAAGTGTGAATGATCTTGACCCGA
GGAAAGCAATCAAGTGCACAACATTGACTTATGATCGAAAACGTTGCGGATATGACAGCT
TGGCTTCCCACCACAGCTGCAAATGTAGCCACAATGAACACTGGCCAGAACACAGGCTCT
GCAAACAGACTTATCTGACTTAATCCCATTCAAAAGTCTCGAGTTATTCGCGTACTATAC
AGATTACAGTTAAGAATTTACCGGGTATCGCCTTGTAGAAGCTTTGCTGAATATCTTCAT
GGTGTTTAGAGAGAAACGCAGCCTCACCCATGTATGCAAGTATCAAACA

Last Updated on 06.12.2018 16:11