Sequences from confirmation amplicon


Confirmation sequences for line 825G08 and hit At5g26290 are shown.
Line ID 825G08
Locus At5g26290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9227678
Primers used for PCR 46xp - 8474, show primer details
   
Sequence Name 38-K119512-0022-825-G08C-46xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 46xp: 5'-GTTTCACTTTACGCGAAGATCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K119512-0022-825-G08C-46xp
GGGAGTGATGTTGATCTCAAACTCTTTGTCTACATGGAAAGCTAACAAATATTTGATTGT
TACAGGTACTTTAAACTGTGAATGGATTTTTATTAGATCCATATATATATTCTAGCGGAA
AACGGTCTATTTCTCCACGATAAGTATGACATCATAACAGATAGAGCCCAGGCTTTAACC
TCGTGCTAGATATGATGTCATATTCTAGCTCTATATGTTATGATGTCATATTCTAGCCAT
TGCTATTTTAAGAAAGTAGACTTCAAATACTAGTCTCATTAGTAAATGAACCGATAGTTG
ATTGTGATTTGCCTTTTTTTTTTTGGCAAAATGGGGAATGGAAAAGGGAACGGGGAAACA
ATCCACCATGGCAACAAAAAATTGGGGTTACGGAAAATTTTTTCCTCATTCAACTTTCTA
CAAGGGAAACAAGGGGTCCCGGGACCAGGACACGGGAACTTTGGGGGCTAAAATCAACTT
CGTAAACCCGCCCCAACAAAAAAAAAAGGTCACTTTCTTATAAACCCCCCCAAACAAGGT
TTTCCCTGGAAAAATACTTCTTTTCTCTCCCTGGAAAAATAAAGTCTATCATTCAAACAA
TTTTCTGGTGGAAAACCAATCCGGGTAAGCCTTTTTCGAACCCGGATTTTTAAAACCAAG
TTCCAATTAATTCTAAAAACCTGGTTACGGTAATGGGATTTTCACGGTATTATGGTATAA
CCGGGAATTTAGGATTAACCCC
   
Sequence Name 06-K119511-0022-825-G08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K119511-0022-825-G08C-8409
TCATTGAATATATTCCGATGCTCTCCTGGAGATACTTTAGCTATTCCTAGTTTCATTCAT
TGTTGTTTTTTTCTCCTGGAGATAAACTTTTATTCTTAGGTTAGTTTTCTTACTATATGT
TGTTACGTGGTTGCTGCTTCGTTGATTCTTTAACCGCAGATTAGTCGCTGCATAAGTGGT
TGTAACAACTGCGTTTGCCTTAAAGCCTTGAGCATATAGGAAGATTGGGACTAAGCCTCC
TTTCGGGTTTAATCCTAATTTCCTGATTATACAATAATACTGTGAAAGTCACATAACAGT
AACAAGCTTTTAAGATTAATTTGGACTTTGGTCTAAAATATCCGGTTACGAAAATGACTA
ACCAGTATCGGTCTCCAACAAGAAACTCGTTAGACTGATAGACTTTATCTTCCAAGGTAG
AGAAATGAAGTATCTTCCAAGTGAAAACATTGTCTGGAGGGTTTGATATGAATGTGACTT
TCTCTTTTTGTTGGGCTGGTTTAACGATGTAGATCTCAGCACCAAATGTACCAGTGTCCT
GCTCACGGTACCCATCGTTCCCGTCGTAGAATGTTGATTGAGGAATAAGTTGTCCGTAAC
CCAACTCTTTTGTCGCATTGTTGTATCGTTTCACTGTACCATCTACAATCCACAATTATG
CAAAAAAAAAAAGGGCAATTCACAATCACTTATGGTTTCTTTTCCA

Last Updated on 06.12.2018 16:11