Sequences from confirmation amplicon


Confirmation sequences for line 826B09 and hit At1g18210 are shown.
Line ID 826B09
Locus At1g18210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6268405
Primers used for PCR 27xs - 8474, show primer details
   
Sequence Name 43-K119832w-0022-826-B09C-27xs
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 27xs: 5'-GTTTAAGGCTATGGGGACTTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K119832w-0022-826-B09C-27xs
ATACGATCGCGACGGTNACATCAACCTCGACGAATTCTCAACTCTCTGTCGCTCGTCTTC
CTCCGCCGCAGAGATCCGTGACGCTTTCGATCTCTACGATCAGGACAAAAACGGACTGAT
CTCCGCCTCTGAGCTTCACCAGGTGCTTAACCGTCTCGGGATGTCTTGCTCTGTTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATTA
TTATAT
   
Sequence Name 19-K119831w-0022-826-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119831w-0022-826-B09C-8409
TTCACAGAGCAGACATCCCGAGACGGTTAAGCACCTGGTGAAGCTCAGAGGCGGAGATCA
GTCCGTTTTTGTCCTGATCGTAGAGATCGAAAGCGTCACGGATCTCTGCGGCGGAGGAAG
ACGAGCGACAGAGAGTTGAGAATTCGTCGAGGTTGATGTAACCGTCGCGATCTGTATCGA
CTTCTTCGAGCACGCGGTTGAGTTCCGTCTCTGTGTAGGAAGTCCCCATAGCCTTAAA

Last Updated on 06.12.2018 16:11