Sequences from confirmation amplicon


Confirmation sequences for line 826H06 and hit At5g14270 are shown.
Line ID 826H06
Locus At5g14270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4606299
Primers used for PCR 35sf - 8474, show primer details
   
Sequence Name 32-K117337-0022-826-H06C-35sf
GenBank Accession LN500739 [GenBank]
Sequencing primer gene specific primer, 35sf: 5'-CCAGGGGATAGTCTAATCACCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117337-0022-826-H06C-35sf
TAACAAGTGAATGTAAAAGGCCTCCTAATTTAAAAATTATAAAATAAGTGCGCTAGCAAC
GTTCACTGCATATCAACAACAATATGGCTCCAAGGCCACTTCTGAGAAAATCTGAGGTCA
CGAGAGTTTGGAAATGACAAATGTAACAACCATACCCCAACAAAAGTTCTATGGAGAACA
AGAATGTCAATATAAACAAGCAAAAGAAAAGATACCATTAGAGTTTCCTAACACCAGATC
CTTCTCTATCGTGACAGGAGAAATGCTGGATGTTGGGTGTTCATTTTCACCAATGTCAAC
AACTTCATCATCCAATTCACTCCCTACAACGAATACAGTAAAAAATGTTACTCTGATTAA
AGAGCCAAGCAAAAAACTTTATCGAACCACAAGCAGGCTGTGAACTAACCATCACAATGT
TGCATTGATGAATTCCCTGGCACTGATCCATGCAGAAGCTGCAATAGATAAAAGTTTCCC
TATTAGCAGTGATGATGCAATTAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 27-K117336-0022-826-H06C-8409
GenBank Accession LN498571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117336-0022-826-H06C-8409
ATATATCTAATCGCATCATCACTGCTAATAGGGAAACTTTTATCTATTGCAGCTTCTGCA
TGGATCAGTGCCAGGGAATTCATCAATGCAACATTGTGATGGTTAGTTCACAGCCTGCTT
GTGGTTCGATAAAGTTTTTTGCTTGGCTCTTTAATCAGAGTAACATTTTTTACTGTATTC
GTTGTAGGGAGTGAATTGGATGATGAAGTTGTTGACATTGGTGAAAATGAACACCCAACA
TCCAGCATTTCTCCTGTCACGATAGAGAAGGATCTGGTGTTAGGAAACTCTAATGGTATC
TTTTCTTTTGCTTGTTTATATTGACATTCTTGTTCTCCATAGAACTTTTGTTGGGGTATG
GTTGTTACATTTGTCATTTCCAAACTCTCGTGACCTCAGATTTTCTCAGAAGTGGCCTTG
GAGCCATATTGTTGTTGATATGCAGTGAACGTTGCTAGCGCACTTATTTTATAATTTTTA
AATTAGGAGGCCTTTTACATTCACTTGTTTACTAACTAAAACTAAATTGCCTTTCGTGTT
CCCTTTATTGATTTTATGGTGATTAGATATTTCCCCT
Primers used for PCR 28tf - 3144, show primer details
   
Sequence Name 22-K117356-0022-826-H06C-28tf
GenBank Accession LN496286 [GenBank]
Sequencing primer gene specific primer, 28tf: 5'-GCCCATAAGGAAAAACATATCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117356-0022-826-H06C-28tf
ACTGTGAGAATGTCGTAGATCCTGCTAAACGGGTTATGACTGATGAGGATAGACTCAAGC
TGGGCAAGGATTTGGAGTCCTTGACTGAGTTCCCGGCACAATTAATAAATTTCTTGAGAG
ATCACAACTCAAATGAAGGAGGGATTGGGGATGATGAGATTGAGATTGACATTAATGATC
TCAGTGACCATGCCTTGTTTCAGTTGCGGGATCTCTTAGACGAGCATTTGCGTGAGATCC
AAAACAAAAAATCAAGTGTTGAACCCTGTGAGATAGAGGTATATCTTCGTCTATACCTGT
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCAATTCAATCCAC
   
Sequence Name 02-K117355-0022-826-H06C-3144
GenBank Accession LN487210 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117355-0022-826-H06C-3144
CGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGG
GATCAGATTGTCGTTTCCCGACAGGTATAGACGAAGATATACCTCTATCTCACAGGGTTC
AACACTTGATTTTTTGTTTTGGATCTCACGCAAATGCTCGTCTAAGAGATCCCGCAACTG
AAACAAGGCATGGTCACTGAGATCATTAATGTCAATCTCAATCTCATCATCCCCAATCCC
TCCTTCATTTGAGTTGTGATCTCTCAAGAAATTTATTAATTGTGCCGGGAACTCAGTCAA
GGACTCCAAATCCTTGCCCAGCTTGAGTCTATCCTCATCAGTCATAACCCGTTTAGCAGG
ATCTACGACATTCTCACAGTCTACTGCAGTGGTCTTCCTCTTCTTAGCCATAGGCACAGG
TATTACGATATG

Last Updated on 06.12.2018 16:11