Sequences from confirmation amplicon


Confirmation sequences for line 828H07 and hit At4g25490 are shown.
Line ID 828H07
Locus At4g25490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13021948
Primers used for PCR JL09 - 8474, show primer details
   
Sequence Name 02-K032598-022-828-H07C-JL09
GenBank Accession LN486922 [GenBank]
Sequencing primer gene specific primer, JL09: 5'-AGTGAGAGAGCCAAACAAGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K032598-022-828-H07C-JL09
CAGCTCGTGCTCACGACGTCGCTGCATTAGCCCTCCGTGGCCGATCAGCATGTCTCAACT
TCGCTGACTCGGCTTGGCGGCTACGAATCCCGGAGTCAACATGCGCCAAGGATATCCAAA
AAGCGGCTGCTGAAGCGGCGTTGGCTTTTCAAGATGAGACGTGTGATACGACGACCACGA
ATCATGGCCTGGACATGGAGGAGACGATGGTGGAAGCTATTTATACACCGGAACAGAGCG
AAGGTGCGTTTTATATGGATGAGGAGACAATGTTTGGGATGCCGACTTTGTTGGATAATA
TGGCTGAAGGCATGCTTTTACCGCCGCCGTCTGTTCAATGGAATCATAATTATGACGGCG
AAGGAGATGGTGACGTGTCGCTTTGGAGTTACTAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTC
   
Sequence Name 02-K032598-022-828-H07C-8409
GenBank Accession LN486921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K032598-022-828-H07C-8409
ATCTCCTTCCCGTCTAATTATGATTCCATTGAACAGACGGCGGCGGTAAAAGCATGCCTT
CAGCCATATTATCCAACAAAGTCGGCATCCCAAACATTGTCTCCTCATCCATATAAAACG
CACCTTCGCTCTGTTCCGGTGTATAAATAGCTTCCACCATCGTCTCCTCCATGTCCAGGC
CATGATTCGTGGTCGTCGTATCACACGTCTCATCTTGAAAAGCCAACGCCGCTTCAGCAG
CCGCTTTTTGGATATCCTTGGCGCATGTTGACTCCGGGATTCGTAGCCGCCAAGCCGAGT
CAGCGAAGTTGAGACATGCTGATCGGCCACGGAGGGCTAATGCAGCGACGTCGTGAGCAC
GAGCTGCCATCTCAGCGGTTTGGAAAGTCCCGAGCCAAATCCTGGTTTTCTTGTTT

Last Updated on 06.12.2018 16:11