Sequences from confirmation amplicon


Confirmation sequences for line 830B04 and hit At3g60110 are shown.
Line ID 830B04
Locus At3g60110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22200175
Primers used for PCR 38uj - 8474, show primer details
   
Sequence Name 61-K118051-0022-830-B04C-38uj
GenBank Accession LN510617 [GenBank]
Sequencing primer gene specific primer, 38uj: 5'-AAAGAATTTCCAAGGAAGACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118051-0022-830-B04C-38uj
GTCGGGACCACTCCTTATTTATAGTGTTAAAACTCTTTTCTCTCTTCTTTTTTTCGATCA
ACACCTTTTCCATTTTCCGAATTACGCTTTATTGTTTCTTATTGTTATTAGTATGAAACG
TATATAACTTTTCATATGGTAGCAAATATCTTTACGATATATATACTATATAGTATTGAA
TATCGATGTTTCCTTGTGTGTTTGCTCTGTAAATTTCACTTCCATATCCGATTATGATTT
TCGTACAGTTGGTAATTTTCTTATGGAATCCCATTTTGTTTATAACGTTTTTCTTGTTAC
CAACACACAAGGTTTTTCGTTGAAGAAATTTGTTTTTTTCGACTCATTTACCATTTTGAT
CACCCAACACTGGACACCTGGCAGCTTCCGGTTGAATCGTGGTTTTGTCGTGTCTTTCAA
GATATGGTCAACTTATTGACTTTTCAGCCGCCTTTAAGCGAGACAAGTTTTTGGGCTTTA
GTTGGGCTTCTAGAAATCCATTACTTATGACGTTTTTATGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 25-K118050-0022-830-B04C-8409
GenBank Accession LN497651 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118050-0022-830-B04C-8409
TACATTGAAATATCATAAAAACGTCATAAGTAATGGATTTCTAGCAGCCCAACTAAAGCC
CAAAAACTTGTCTCGCTTAAAGGCGGCTGAAAAGTCAATAAGTTGACCATATCTTGAAAG
ACACGACAAAACCACGATTCAACCGGAAGCTGCCAGGTGTCCAGTGTTGGGTGATCAAAA
TGGTAAATGAGTCGAAAAAAACAAATTTCTTCAACGAAAAACCTTGTGTGTTGGTAACAA
GAAAAACGTTATAAACAAAATGGGATTCCATAAGAAAATTACCAACTGTACGAAAATCAT
AATCGGATATGGAAGTGAAATTTACAGAGCAAACACACAAGGAAACATCGATATTCAATA
CTATATAGTATATATATCGTAAAGATATTTGCTACCATATGAAAAGTTATATACGTTTCA
TACTAATAACAATAAGAAACAATAAAGCGTAATTCGGAAAATGGAAAAGGTGTTGATCGA
AAAAAAGAAGAGAGAAAAGAGTTTTAACACTATAAAATAAGGAGTGGTCCCGACCAAATT
GGACAATGTGTGGATAATTTCTCATAATTTTATTGGTCTTCCT

Last Updated on 06.12.2018 16:11