Sequences from confirmation amplicon


Confirmation sequences for line 830D08 and hit At3g26220 are shown.
Line ID 830D08
Locus At3g26220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9596749
Primers used for PCR DA41 - 8474, show primer details
   
Sequence Name 59-K108477-0022-830-D08C-DA41
GenBank Accession LN510021 [GenBank]
Sequencing primer gene specific primer, DA41: 5'-TGAAGACAAAACAAATCAAGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K108477-0022-830-D08C-DA41
TTTATAACAGAACAAGATGAATCTTTCAACTCACCATTGATCATCTCAAAGGAATTATCC
AAGTAAGCATAAAATCTAGTTCACAGTTCTGTCAAAACTCAGGGACTTTTATCTCTAAAG
TATCTTATCTCTTAAGTTAAATATGTTCTTAACCATCTTGTGTCATTGTTGACCTAGAAT
ATATATCTTGCTGGAGTAGACACAAGTGCCATCACCATGATTTGGGCAATGGCAGAGCTT
GTTAATGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 43-K108476-0022-830-D08C-8409
GenBank Accession LN504862 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K108476-0022-830-D08C-8409
TCGGACATGAGCCATTTACATTGAATTATCCTGCCATTAACAAGCTCTGCCATTGCCCAA
ATCATGGTGATGGCACTTGTGTCTACTCCAGCAAGATATATATTCTAGGTCAACAATGAC
ACAAGATGGTTAAGAACATATTTAACTTAAGAGATAAGATACTTTAGAGATAAAAGTCCC
TGAGTTTTGACAGAACTGTGAACTAGATTTTATGCTTACTTGGATAATTCCTTTGAGATG
ATCAATGGTGAGTTTGAAAGATTCATCTTGTTCTTGTTTATAAATAGTCTCTAAGATCGA
GTCGATGATATCAGGGCGATCTTGATTTGTTTTGTCTTC

Last Updated on 06.12.2018 16:11