Sequences from confirmation amplicon


Confirmation sequences for line 831C09 and hit At5g61690 are shown.
Line ID 831C09
Locus At5g61690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24790985
Primers used for PCR 62lu - 8474, show primer details
   
Sequence Name 31-K113982-0022-831-C09C-62lu
GenBank Accession LN500249 [GenBank]
Sequencing primer gene specific primer, 62lu: 5'-AAACAACCATCTTAAAATTAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113982-0022-831-C09C-62lu
ATTTAAGAGAATGTAGCAGTTGACATGCCGGTTCTCTAGGAAGACCAGCTTGGGATCGCC
GATAAGTGCTACCGCAACACTGAGTCGACGTTTCATTCCACCACTGTAACTTCCTGCTCT
AACTTTCGCCGCTCCTGTCAGTTTTACATCCGCCAGTAACTTCTCAGCAGTCTATAACAG
AAACGAAACTTGTAAGATAAGTTGCATGGATTGGAAAGTTCAATAAAATCATCAGACCTC
TTTGCACTTTGAGATTTTTCAGTCGGCCTACCGATTTGATCGATGCTGGTGGCAACCCTT
TGATGCTAGCAAAGAGGTGGAGGTGTTGTTCACTAGACAAAGCATCCCAAAGAATATCAA
ACTATAAAAAAAGAAAAACGTGTAAGAGATGATTGTATAAGAGACAATGCTACATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K113981-0022-831-C09C-8409
GenBank Accession LN493059 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113981-0022-831-C09C-8409
CTCTATACATCATCTCTTACACGTTTTTCTTTTTTTAAGTTTGATATTCTTTGGGATGCT
TTGTCTAGTGAACAACACCTCCACCTCTTTGCTAGCATCAAAGGGTTGCCACCAGCATCG
ATCAAATCGGTAGGCCGACTGAAAAATCTCAAAGTGCAAAGAGGTCTGATGATTTTATTG
AACTTTCCAATCCATGCAACTTATCTTACAAGTTTCGTTTCTGTTATAGACTGCTGAGAA
GTTACTGGCGGATGTAAAACTGACAGGAGCGGCGAAAGTTAGAGCAGGAAGTTACAGTGG
TGGAATGAAACGTCGACTCAGTGTTGCGGTAGCACTTATCGGCGATCCCAAGCTGGTCTT
CCTAGATGAACCGGCATGTCAACTTGCTACATTCTTCTTTAAATATGTGTCTATATATAA
AAACAATTAATTTTAAATGGGTTGTT

Last Updated on 06.12.2018 16:11