Sequences from confirmation amplicon


Confirmation sequences for line 831D03 and hit At4g11380 are shown.
Line ID 831D03
Locus At4g11380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6922665
Primers used for PCR 55kh - 8474, show primer details
   
Sequence Name 51-K113817-0022-831-D03C-55kh
GenBank Accession LN507567 [GenBank]
Sequencing primer gene specific primer, 55kh: 5'-ATGGATAAGCTTTTGGTAGCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113817-0022-831-D03C-55kh
GGGTCAGTTTTTAACTAGATGCTTTATCGAAGTATAAAGCAGCTGATCCCCGTGAGGCTG
AAAATATTGTTGAGAGAGTAACACCAAGGTTGCAACATGCCAACTGCGCTGTTGTTCTTT
CAGCTGTTAAGGTGAGCGACTTTCATTGATTGTTATCCTCTCTACTTCTAGTTGGTAGCT
CGTCAAACTTGATTGTAAAACATCAGGCATCATCAAATGCCTTTGCATATTTTCCTATTG
CGTTCTCACTTCTGTTTCATATTTGGTAATTGCCTGACAGATGATCCTTCAACAAATGGA
ACTTATTACTAGTACAGACGTGATTAGAAATCTTTGTAAGAAGATGGCTCCACCCCTTGT
TACATTGCTTTCTGCCGAACCTGAGATACAATATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 25-K113816-0022-831-D03C-8409
GenBank Accession LN497532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113816-0022-831-D03C-8409
GTTCGGCAGAAGCAATGTAACAAGGGGTGGAGCCATCTTCTTACAAAGATTTCTAATCAC
GTCTGTACTAGTAATAAGTTCCATTTGTTGAAGGATCATCTGTCAGGCAATTACCAAATA
TGAAACAGAAGTGAGAACGCAATAGGAAAATATGCAAAGGCATTTGATGATGCCTGATGT
TTTACAATCAAGTTTGACGAGCTACCAACTAGAAGTAGAGAGGATAACAATCAATGAAAG
TCGCTCACCTTAACAGCTGAAAGAACAACAGCGCAGTTGGCATGTTGCAACCTTGGTGTT
ACTCTCTCAACAATATTTTCAGCCTCACGGGGATCAGCTGCTTTATACTTCGATAAAGCA
TCTAGTATAAAAACTTGACCCCACCTGCGATGATCATGGTCAGTACTAAAAATGAAGCTA
CCAAAAGCTTATCCA

Last Updated on 06.12.2018 16:11