Sequences from confirmation amplicon


Confirmation sequences for line 832C09 and hit At4g10170 are shown.
Line ID 832C09
Locus At4g10170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6345095
Primers used for PCR 16jk - 8474, show primer details
   
Sequence Name 90-K113193-0022-832-C09C-16jk
GenBank Accession LN515053 [GenBank]
Sequencing primer gene specific primer, 16jk: 5'-TCTTGCTGCTCTGTGTTTAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K113193-0022-832-C09C-16jk
GTAAAGAGATTTGGGTTTTTATTGGAGATGGGTTTGTTTATTTCGCCATTGTTGATGAGG
TTTTGAAGAGATCTAGTGTTCTTAAGTTTCTTGAACATTTGAGAGATGAATTTAAGAAAG
CTGCTAGGGAGAATTCTAGAGGAAGTTTTACTGCTATGATTGGTTCTATTAATGTTGAAG
ATCAGCTTGTTCCTGTTGTTACTAGACTTATAGCTTCTCTTGAACGTGTTGCTGAGAGTA
GTAGTAACAATGAATTGAAGAGTAGTAATCTTGGTGAACAAAGCGAAGGTTCGAATTCTA
CTAAAGCTCCGTTGTTGGGGAGGTTAAGTAAGCAAGAGAAGAAGAAAGGAATGAATCAGC
AAGATCAATTGCCCAATTCAAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 53-K113192-0022-832-C09C-8409
GenBank Accession LN508209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113192-0022-832-C09C-8409
TTGAATATATCTTGAATTGGGCAATTGATCTTGCTGATTCATTCCTTTCTTCTTCTCTTG
CTTACTTAACCTCCCCAACAACGGAGCTTTAGTAGAATTCGAACCTTCGCTTTGTTCACC
AAGATTACTACTCTTCAATTCATTGTTACTACTACTCTCAGCAACACGTTCAAGAGAAGC
TATAAGTCTAGTAACAACAGGAACAAGCTGATCTTCAACATTAATAGAACCAATCATAGC
AGTAAAACTTCCTCTAGAATTCTCCCTAGCAGCTTTCTTAAATTCATCTCTCAAATGTTC
AAGAAACTTAAGAACACTAGATCTCTTCAAAACCTCATCAACAATGGCGAAATAAACAAA
CCCATCTCCAATTAAAAACCCAAATCTTCTTTTACCAATAGTTTCAAAGTACCAAGTATG
AAAAGGAGGAGACTTTTCTAAACACAGAGCAGCAAG

Last Updated on 06.12.2018 16:11