Sequences from confirmation amplicon


Confirmation sequences for line 832E11 and hit At1g55150 are shown.
Line ID 832E11
Locus At1g55150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20576257
Primers used for PCR 37ew - 8474, show primer details
   
Sequence Name 61-K110987-0022-832-E11C-37ew
GenBank Accession LN510549 [GenBank]
Sequencing primer gene specific primer, 37ew: 5'-GTGGATATAGGTGTGGAGATCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K110987-0022-832-E11C-37ew
ACAACAAAACCTACGAAGGGTTACTTATCTTGTTTTGGATGAGGCTGATCGAATGCTTGA
TATGGGGTTTGACCCTCAGATCCGGAAAATTGTTTCACATGTTCGTTTACTTACTCCTGT
CTTCGTCATATATATGTGTATGCTAGTTGCTTTGTCTATAACCTTTCTTTTTAAACCTGT
GCAGATTCGGCCAGACCGTCAAACTTTGTACTGGAGTGCCACTTGGCCTAAGGAGGTGGA
ACAACTATCGAAGAAGTTTCTTTATAACCCATACAAAGTAAGAGAGTAAGAGACATCTCT
CTAACGTTTCTCACAGTGGTTTAGTTGTCAACAAAAACTTATCAGGCTTCTTTGGTCTTA
ATTGCAGGTGATAATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTACAGCACGTTATTA
   
Sequence Name 45-K110986-0022-832-E11C-8409
GenBank Accession LN505564 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K110986-0022-832-E11C-8409
ATTTACATTGAATATATCCTATTATCACCTGCAATTAAGACCAAAGAAGCCTGATAAGTT
TTTGTTGACAACTAAACCACTGTGAGAAACGTTAGAGAGATGTCTCTTACTCTCTTACTT
TGTATGGGTTATAAAGAAACTTCTTCGATAGTTGTTCCACCTCCTTAGGCCAAGTGGCAC
TCCAGTACAAAGTTTGACGGTCTGGCCGAATCTGCACAGGTTTAAAAAGAAAGGTTATAG
ACAAAGCAACTAGCATACACATATATATGACGAAGACAGGAGTAAGTAAACGAACATGTG
AAACAATTTTCCGGATCTGAGGGTCAAACCCCATATCAAGCATTCGATCAGCCTCATCCA
AAACAAGATAAGTAACCCTTCGTAGGTTTGTGTTGTTCGACTCCATCATGTCTATTAACC
TCCCAGGAGTAGCTATAACGATCTCCACACCTATNTCCA

Last Updated on 06.12.2018 16:11