Sequences from confirmation amplicon


Confirmation sequences for line 832F07 and hit At5g58700 are shown.
Line ID 832F07
Locus At5g58700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23715236
Primers used for PCR DN01 - 8474, show primer details
   
Sequence Name 44-K101830-0022-832-F07C-8409
GenBank Accession LN505132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K101830-0022-832-F07C-8409
GCCTTTACATTGAATAATCCTCCAACAATGTGAAGAATTGAGAATCTTCTCTCTGCAGAA
GTAAGCAACTACTAGAATAGAAAGTATCCAAAATAGAAACTTCTAAATTTAGACAGGTTA
ATCAAACAGTCAGATTCCAGAATTTCAGCAGCAGCTGCAGAGTACACACCAAGAACATAT
CATCGAGAAAAGTTTCACCCTTTTATTGGGATTTGATGAATTATTCATAAGCATTAAGAA
GAAGCTACCAAAAGTGATGATTTTTATTTTCTCTTTGATCAAACTAAATTTAGGCCAGAA
AATAGCAAAAAGAGAAAAGAGTCAAATAGCGATGAGAGAGAAAACATTCTGAGATTTTCC
GGTAAAACCAACAGAGAGAAGAATCTAAAGTCGTAATCTTTCAATCTCAATCTTTATCAA
GAAACAAACACCATCAAATCCCAGATTCCAAAAAGTCTCAGACTTTTCTTCCCAGGAACA
AAAAAAAAAAAAAAATTTGGACTTGAAAATTTTATAACCCTGATCGGCAATGGGAGGATT
AAAATCAGTAAAAAAGAGGTAATATTTGAATTCTTCAAAAGTAGGGTTT
   
Sequence Name 88-K101833-0022-832-F07C-DN01
GenBank Accession LN514847 [GenBank]
Sequencing primer gene specific primer, DN01: 5'-TTCACCAGACGAAACCTTACTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K101833-0022-832-F07C-DN01
TCTAATCCTCCATCGCCGATCAGGTTTATAAATTTTTCAAGTCAAACTCTTTTTTTTTTT
TTTGTCCCGGGAAAAAAAATTCGAAAACTTTTGGAAACCGGGAATTTGAGGGGGTTTGTT
CCTGGAAAAAAATGGAAATTGAAAAATTCCAACTTTAAATCCCCCCCCCGGTGGGTTTTC
CCGAAAAACCCCAAAAGGTTTTCCCCCCCACCCCTTTTGAACCCTTTCCCCTTTTGGCAA
TTTTCGGGCCAAAATTTATTTGGACCAAAAAAAAAAAAAAAACCCCCCCTTTGGGAACCT
CCTCCTAAAGGCTAAAAAAAAATCCACCAAACCCCAAAAAAGGGGGAAAACTTTCCCCAA
AAAAATGTCCTGGGGGGGAACCCCGCACCTCCGGCGAAAATTCGGGAACCGAACGGTTTG
ATAAACCGGCCAAAATTAAAAATTTCCTATTTGGAAAACTTTCAATCCAAGAAGTGGCTA
ACTTCGGCAAAAAAAAAATCCCCAATTCTCCACATTGTGGGAGGAAATATCCAATTGAAA
AGGGCTCCAGGCCCGGAAAACCTCCTGGGACCACCAAGGAGTATAAGGGCCAATAGGGAA
AAAAAAAAAAAGTATTTCCCATTTTTTTTCCAATTCAAAAAAGAAAATC

Last Updated on 06.12.2018 16:11