Sequences from confirmation amplicon


Confirmation sequences for line 832H12 and hit At3g51110 are shown.
Line ID 832H12
Locus At3g51110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18984126
Primers used for PCR DA42 - 8474, show primer details
   
Sequence Name 40-K106288-0022-832-H12C-8409
GenBank Accession LN503717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K106288-0022-832-H12C-8409
GCCATTTCAATTGAATATACGCGAGGTAGAATCTTCACGGCTCGATCCCAAACGTTCCTC
GCATGGTTGACGGATTTGTTCCTCATCTCGAACTCTGCGTATTTGAGCCAGAGCGTATGG
TTCCTGTAACTCTCGTCTTCCAAAGCTCGTTCCCACACGCTCCGAGCACGATCGTGATCC
TTCTGAGACTCTTCCCAATCAGCATACCTCACCCAAACTTGTGAGTTCGTTTTTGCGCCG
CGGATCTGGTCCTCAAACTCCTTCCGACGTCGGAGTCTGTACTCGGCAAGCTCATCCGAA
TCTGTGATCTTCTGTTTAGGCGGCCGCAGGATGCGACTGTCCTCTCTCTCTCTCGCCTCC
TTCAAAACCTGCTCCGCCGTGATTTGAACCGGAGCAGGGGTTTTGTTCTTAACCATCCTT
GGAATCTCANTCATCTT
   
Sequence Name 40-K106289-0022-832-H12C-DA42
GenBank Accession LN503718 [GenBank]
Sequencing primer gene specific primer, DA42: 5'-GAAGATGATGATGATTCCAAGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K106289-0022-832-H12C-DA42
TCGGTTAATCCGGCGGAGCAGGTTTTGAAGGAGGCGAGAGAGAGAGAGGACAGTCGCATC
CTGCGGCCGCCTAAACAGAAGATCACAGATTCGGATGAGCTTGCCGAGTACAGACTCCGA
CGTCGGAAGGAGTTTGAGGACCAGATCCGCGGCGCAAAAACGAACTCACAAGTTTGGGTG
AGGTATGCTGATTGGGAAGAGTCTCAGAAGGATCACGATCGTGCTCGGAGCGTGTGGGAA
CGAGCTTTGGAAGACGAGAGTTACAGGAACCATACGCTCTGGCTCAAATACGCAGAGTTC
GAGATGAGGAACAAATCCGTCAACCATGCGAGGAACGTTTGGGATCGAGCCGTGAAGATT
CTACCTCGCGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11