Sequences from confirmation amplicon


Confirmation sequences for line 833A10 and hit At5g50260 are shown.
Line ID 833A10
Locus At5g50260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20455880
Primers used for PCR 14jk - 8474, show primer details
   
Sequence Name 91-K113193-0022-833-A10C-14jk
GenBank Accession LN515128 [GenBank]
Sequencing primer gene specific primer, 14jk: 5'-GAACTCAAAAGCAAGGTCCATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K113193-0022-833-A10C-14jk
AATCTACTAGCTCTTGCTCTGAGAGTGATGTCAGCTTTTTAGTTCTTATTTGGTTGATCC
CTTCCACCGCAACAACTGTTGAAAACGCCCAACAACTCCCTGTAATAACCACAAACCAAA
TCTGTGTTAATTCTCATTTTCCATATATCTTTTAGATTGAAGGATCAATGAAAAGAAGAG
TTTTACCGCATTGGCCTTGGTTTTTGACAGGAGTGACGGCTCCATTCTTTCTCCAATCAA
CGGAGGTTGGGAGAGTATTTACATTTGCATACATGAAGCTCTTTGTGGCCTTTTTTTCGC
CTTGAAACATCCTATGATGTTTGATGTTTGAACCAGCGTATGTTCTCCTAAATTCTTCAC
TAGTCATGTCACCAAACTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 54-K113192-0022-833-A10C-8409
GenBank Accession LN508536 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113192-0022-833-A10C-8409
GATTTAGGAGAACATACGCTGGTTCAAACATCAAACATCATAGGATGTTTCAAGGCGAAA
AAAAGGCCACAAAGAGCTTCATGTATGCAAATGTAAATACTCTCCCAACCTCCGTTGATT
GGAGAAAGAATGGAGCCGTCACTCCTGTCAAAAACCAAGGCCAATGCGGTAAAACTCTTC
TTTTCATTGATCCTTCAATCTAAAAGATATATGGAAAATGAGAATTAACACAGATTTGGT
TTGTGGTTATTACAGGGAGTTGTTGGGCGTTTTCAACAGTTGTTGCGGTGGAAGGGATCA
ACCAAATAAGAACTAAAAAGCTGACATCACTCTCAGAGCAAGAGCTAGTAGATTGTGATA
CAAACCAGAACCAAGGATGCAATGGAGGTCTAATGGACCTTGTTNTTGAGTT

Last Updated on 06.12.2018 16:11