Sequences from confirmation amplicon


Confirmation sequences for line 833F02 and hit At3g52310 are shown.
Line ID 833F02
Locus At3g52310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19400021
Primers used for PCR BF03 - 8474, show primer details
   
Sequence Name 65-K028751-022-833-F02C-8409
GenBank Accession LN511312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K028751-022-833-F02C-8409
CCATTTACATTGAATAATCCTGGCCAATTGAATATTTCAAATACACCTTTTGATCATTTT
CCCATGTTTCCCATAAGATTATCTATCTTCGGAAATGGGTTACCTTCAGATATATCGGAA
AGGTTGGTTCTGCCTGAAACTTGACCACGCTACTGGTTGCTGCTTCAATATCTTCCACTG
CAATATGAATGATACCAGAACAAGAACCCAATAAAGTCCAAAGTTAACATCAAGGCTTCA
ATCATGTGCGGTATCTAATGGTTTTCTTACAAATATCGTCATTGCTACAGCGCTCTGAAG
CAATTATCTCTTCGTGTGGCACGGTGAATCCGGTGAAAGAGAAGGATAGTCCAAGAGAAG
CACTAGAGG
   
Sequence Name 65-K028751-022-833-F02C-BF03
GenBank Accession LN511313 [GenBank]
Sequencing primer gene specific primer, BF03: 5'-GCCTCTAGTGCTTCTCTTGGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K028751-022-833-F02C-BF03
ATAATTGCTTCAGAGCGCTGTAGCAATGACGATATTTGTAAGAAAACCATTAGATACCGC
ACATGATTGAAGCCTTGATGTTAACTTTGGACTTTATTGGGTTCTTCTTCTGGTATCATT
CATATTGCAGTGGAAGATATTGAAGCAGCAACCAGTAGCGTGGTCAAGTTTCAGGCAGAA
CCAACCTTTCCGATATATCTGAAGGTAACCCATTTCCGCTGATAGATAATCTTATGGGAA
ACATGGGAAAATGATCTTTATATCTATTTGAAATATTCAATTGGCCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTC
Primers used for PCR FN40 - 8474, show primer details
   
Sequence Name 22-K035334-022-833-F02C-8409
GenBank Accession LN496020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K035334-022-833-F02C-8409
TGAGCCACACCAAAAAGTCCGTAGAAATATATAAAATTTTGCTGCCATTGGTCAGTCCAA
ACTTCAATGGGTCTGTTGTATCTCATATGATGTTTCCTAAACAAGAAATCGCTATTTCTC
CCATAGTGAAGAAACATCCATAAATTTTACAAGTAGAATAATCTTCATCATTGCATTAAG
ACATTTGGAAACCTCTGTTTTGCCTTCTTTGTTGGTCTAACTTTGGTGCCCAAAAGTCAA
GCTTAGGAGGCTCAACTACCATTTACTTGTTATCGGCAACATGTGAAGCAGAGCTTCACG
CTGGACCAAAAGGGTATGGTTATATTAGTATCGCCTTGTATAAATCAGAGAGAAATTCTA
GTCATATTTCTATGGAAAAAGACTGGTTTTCATGAAAATTCAAGCATATAGGAACTTTTA
CTTGCAATTCTTTCTGACCAAATCAATTCTTATCATGTGTTGTGCAGTTCATAGATATCA
CATACAAGGTGACAACCAAAGGAATGACATCTTCA
   
Sequence Name 22-K035334-022-833-F02C-FN40
GenBank Accession LN496021 [GenBank]
Sequencing primer gene specific primer, FN40: 5'-TCTGATGAAGATGTCATTCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K035334-022-833-F02C-FN40
TGAATCTATGACTGCACAACACATGATAAGAATTGATTTGGTCAGAAAGAATTGCAAGTA
AAAGTTCCTATATGCTTGAATTTTCATGAAAACCAGTCTTTTTCCATAGAAATATGACTA
GAATTTCTCTCTGATTTATACAAGGCGATACTAATATAACCATACCCTTTTGGTCCAGCG
TGAAGCTCTGCTTCACATGTTGCCGATAACAAGTAAATGGTAGTTGAGCCTCCTAAGCTT
GACTTTTGGGCACCAAAGTTAGACCAACAAAGAAGGCAAAACAGAGGTTTCCAAATGTCT
TAATGCAATGATGAAGATTATTCTACTTGTAAAATTTATGGATGTTTCTTCACTATGGGA
GAAATAGCGATTTCTTGTTTAGGAAACATCATATGAGATACAACAGACCCATTGAAGTTT
GGACTGACCAATGGCAGCAAAATTTTATATATTTCTACGGACTTTTTGGTGTTGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTC
Primers used for PCR FN40 - 8474, show primer details
   
Sequence Name 46-K118471-0022-833-F02C-FN40
GenBank Accession LN506011 [GenBank]
Sequencing primer gene specific primer, FN40: 5'-TCTGATGAAGATGTCATTCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118471-0022-833-F02C-FN40
ACACACATGATAAGAATTGATTTGGTCAGAAAGAATTGCAAGTAAAAGTTCCTATATGCT
TGAATTTTCATGAAAACCAGTCTTTTTCCATAGAAATATGACTAGAATTTCTCTCTGATT
TATACAAGGCGATACTAATATAACCATACCCTTTTGGTCCAGCGTGAAGCTCTGCTTCAC
ATGTTGCCGATAACAAGTAAATGGTAGTTGAGCCTCCTAAGCTTGACTTTTGGGCACCAA
AGTTAGACCAACAAAGAAGGCAAAACAGAGGTTTCCAAATGTCTTAATGCAATGATGAAG
ATTATTCTACTTGTAAAATTTATGGATGTTTCTTCACTATGGGAGAAATAGCGATTTCTT
GTTTAGGAAACATCATATGAGATACAACAGACCCATTGAAGTTTGGACTGACCAATGGCA
GCAAAATTTTATATATTTCTACGGACTTTTTGGTGTTGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 14-K118470-0022-833-F02C-8409
GenBank Accession LN492721 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118470-0022-833-F02C-8409
AGCCACACAAAAAGTCCGTAGAAATATATAAAATTTTGCTGCCATTGGTCAGTCCAAACT
TCAATGGGTCTGTTGTATCTCATATGATGTTTCCTAAACAAGAAATCGCTATTTCTCCCA
TAGTGAAGAAACATCCATAAATTTTACAAGTAGAATAATCTTCATCATTGCATTAAGACA
TTTGGAAACCTCTGTTTTGCCTTCTTTGTTGGTCTAACTTTGGTGCCCAAAAGTCAAGCT
TAGGAGGCTCAACTACCATTTACTTGTTATCGGCAACATGTGAAGCAGAGCTTCACGCTG
GACCAAAAGGGTATGGTTATATTAGTATCGCCTTGTATAAATCAGAGAGAAATTCTAGTC
ATATTTCTATGGAAAAAGACTGGTTTTCATGAAAATTCAAGCATATAGGAACTTTTACTT
GCAATTCTTTCTGACCAAATCAATTCTTATCATGTGTTGTGCAGTTCATAGATATCACAT
ACAAGGTGACAACCAAAGGAATG

Last Updated on 06.12.2018 16:11