Sequences from confirmation amplicon


Confirmation sequences for line 834D04 and hit At5g22910 are shown.
Line ID 834D04
Locus At5g22910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7662026
Primers used for PCR 04iv - 8474, show primer details
   
Sequence Name 52-K113214-0022-834-D04C-8409
GenBank Accession LN507875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113214-0022-834-D04C-8409
CAATTGAATATATCCTGATTATTTACGTGCTATTGAACGCGGGGATTTTGCCTACGATTA
TAAAAGCACTATATGATCCAAAGAGGAAGTATATTGGATATGTCAAGAGAGACATTATGC
ATCTTAAAACAAACTCAGATCTCAAGATTCTAACTTGTTTGCACAAGCCTGACAACATCT
CAGGTGCGATCTCGCTACTTGAATTGCTTTCCTCGCCGCTAAACAACGATAACAAAGACA
GAGGAGTCATTGCTGTCACGGCGTTACACCTAGTGAAACTCGCAGGACGTACGTTTCCCA
TCTTGATACCTCATGACAAGCGGTCTAAAGCGCGGTTGCTTCAAAACTCTTACATACAAA
CAATGATGCTCGCGTTCACCGAGTTTCAACAAGAGAATTGGGAATCTACCACTGTTAGTT
CCTTCACAGCTTATTCTCATGAGAACCTAATGGATCAAGACATTTG
   
Sequence Name 52-K113215-0022-834-D04C-04iv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 04iv: 5'-GCAAATGTCTTGATCCATTAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113215-0022-834-D04C-04iv
TCGTAGGGGCAAANNATCCCCGNCNNGTTCAATANNGCACGTAANANNTAATCAGGGAAT
ATATNNTCAATTGNNTAAATGGCNNTTCATGTCCGGGGGAANNATCTACATGGNNNATCA
GCAATGNNNAGTATGATNNGGTCAATATNNGGAGAANNAAAGAAAGAAAGTANNATTACC
AANNTTTTTTTTNNCAATTCAAAAATGGTAGAAATGTCCGCA

Last Updated on 06.12.2018 16:11