Sequences from confirmation amplicon


Confirmation sequences for line 834F08 and hit At2g19410 are shown.
Line ID 834F08
Locus At2g19410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8407648
Primers used for PCR 46sj - 8474, show primer details
   
Sequence Name 49-K116518-0022-834-F08C-46sj
GenBank Accession LN506958 [GenBank]
Sequencing primer gene specific primer, 46sj: 5'-TTCGGTCTAGGAGAACAAAAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116518-0022-834-F08C-46sj
GTGAAGTATACATTGTGTGTAAAGACAGAATCACTACAAAATCTATGGATCCATTAATCA
ACCGAGGTAAAAATTAGGATTTTGTGTTTCGTTTTTTTTTTAAAGAAAATTATTGTTTAA
TACTACATCCATAGTTGATGTAATTTCTTAAACATGGTAATTTATAGGCAAAACATTTTA
TTTGATAACACAATATTTTCTCATTTCTTAAAACTTCTCTTTAATCGTGGTAACCAGAGC
CGTGCACAAGCCGTTTCAAAACCGGTATAACCTCTGATTTAAGATCCGGTCTATCTCGGC
ATCGAAATTCAGCACATTTTAAACCGATCCGAGCCAATTCCTCGGATCGGTTACCACGAT
TAAATTCACAAATAAATTCACGGCGAATTCATGTAAATTCACTAAATATACTAAATTTGA
GTTACTAAATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAAAAA
   
Sequence Name 23-K116517-0022-834-F08C-8409
GenBank Accession LN496709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116517-0022-834-F08C-8409
GCCTTTACATTGAATTATCCTGTAAATTTAGTAACTCAAATTTAGTATATTTAGTGAATT
TACATGAATTCGCCGTGAATTTATTTGTGAATTTAATCGTGGTAACCGATCCGAGGAATT
GGCTCGGATCGGTTTAAAATGTGCTGAATTTCGATGCCGAGATAGACCGGATCTTAAATC
AGAGGTTATACCGGTTTTGAAACGGCTTGTGCACGGCTCTGGTTACCACGATTAAAGAGA
AGTTTTAAGAAATGAGAAAATATTGTGTTATCAAATAAAATGTTTTGCCTATAAATTACC
ATGTTTAAGAAATTACATCAACTATGGATGTAGTATTAAACAATAATTTTCTTTAAAAAA
AAAACGAAACACAAAATCCTAATTTTTACCTCGGTTGATTAATGGATCCATAGATTTTGT
AGTGATTCTGTCTTTACACACAATGTATACTTCACATGTTTCTGGTGCGTATCTTAAAAC
GGTCAGTGGTACTCCTGTACCTTTTGTTCTCCTAGACCGAAA

Last Updated on 06.12.2018 16:11