Sequences from confirmation amplicon


Confirmation sequences for line 835A09 and hit At1g53830 are shown.
Line ID 835A09
Locus At1g53830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20100226
Primers used for PCR JF32 - 8474, show primer details
   
Sequence Name 37-K102175-0022-835-A09C-8409
GenBank Accession LN502563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102175-0022-835-A09C-8409
GTCTTCGGTTTCTTGAAAGAATTGACGATTTGAGTGGACATAAAGTGTGTCTTGATAAGC
AAACATGTCGCATTGGTAAAAAGCGGAAAAATCTGATCCGACTCGGAGAGCCACCGCTTG
ATGCTTCGACGGACCGGCGGTGTTTTGGAATGTGATGTCACGCGCTAGAAACCTCTCGCC
GACGGCAGCTACAGTCAATAGAAAACAGTACTATCATTTAACCGTGGAAATGAGTTACGA
TCGTGTCTACGTTATCATCTACCAACAAAACGTTACACATGCGTCATTATTACATTCTTT
TTACATACAAACAAATGTGTATGTCAGTATGTGTTGTTAGCTATTACGTGATACTACTGT
TTACTTAATTCCTTAAAAATAAAATCAAAGAAGAACAGTATTTACCAACAGTAGCGGAGT
GGAAAGTGGTGCTTCCATCGACGACNTTTCTACTTCCAG
   
Sequence Name 37-K102179-0022-835-A09C-JF32
GenBank Accession LN502564 [GenBank]
Sequencing primer gene specific primer, JF32: 5'-ACTGGAAGTAGAAATGTCGTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102179-0022-835-A09C-JF32
TAATACTGTTCTTCTTTGATTTTATTTTTAAGGAATTAAGTAAACAGTAGTATCACGTAA
TAGCTAACAACACATACTGACATACACATTTGTTTGTATGTAAAAAGAATGTAATAATGA
CGCATGTGTAACGTTTTGTTGGTAGATGATAACGTAGACACGATCGTAACTCATTTCCAC
GGTTAAATGATAGTACTGTTTTCTATTGACTGTAGCTGCCGTCGGCGAGAGGTTTCTAGC
GCGTGACATCACATTCCAAAACACCGCCGGTCCGTCGAAGCATCAAGCGGTGGCTCTCCG
AGTCGGATCAGATTTTTCCGCTTTTTACCAATGCGACATGTTTGCTTATCAAGACACACT
TTATGTCCACTCAAATCGTCAATTCTTTTCAATGAAACCGAAGACATGCAGCTGATAGAC
ATGTAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
Primers used for PCR 08au - 3144, show primer details
   
Sequence Name 13-K106868-0022-835-A09C-08au
GenBank Accession LN492059 [GenBank]
Sequencing primer gene specific primer, 08au: 5'-CATTGACTTGGTTCAACTTGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106868-0022-835-A09C-08au
ATTATGATCATGTAGATGAGAAATACAATGTTTATTAAAATAATAAGTCAATCTTTTAAT
TTCAAAGACTTAACGAGAAAGGAAAACCGGTCGACGCTAACCAGCCTCCACCACCAATAA
ACTGACCAGCCGTAAATGGCTGAGCTTCTGTATCACTTGTAATTACCTTATAGCCCTTCC
ACTTCACTCTATTTGCAGTTCCTGCCCCTCCTCCTCTATTCAAATACTCTCTATAAGTTA
GAGTGTCCAACGCAAAACTCCCGCTCCACTCATGCCATCCTTCGGGTCGGATCACGTCGG
ATATATCCGATTGCATTATCACCGTCCGTGAATATTCCTTCCAAGGACGACCAAGATACG
TCGGGAACGTTCCTTTCACGGCCAAAAGATCCGACGTGCCACCAATACGACAATTCTGGA
TCACAATCCCCGTGTTTTGATTCGGATCAGACCGTCCTTGGGCGGTAACCATGTTTTTCT
GACCGGAATTTGGTCTGCGTGCATTTATATCGCAGTCTTGTAAAACCGCGGCTGCGTTTC
CGAAGATGAAATCGACGGTTCCGGTTATGTCCGGGAAATCTACATGGACCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGC
CTTGATTTCGCCATTCCCAGATACCCATTTCATCTTCAGATTGGTCTGAGATTATGCGAA
AATATACACTCATATACATAAATACTGACAGTTTGAGCTACCAATTCAGTGTAGCCCATT
ACCTTACATAATTCACTCAAATGCTAGGCAGTCTGTCACCTCGGCGTC

Last Updated on 06.12.2018 16:11