Sequences from confirmation amplicon


Confirmation sequences for line 835G07 and hit At1g14370 are shown.
Line ID 835G07
Locus At1g14370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4917750
Primers used for PCR BH53 - 8474, show primer details
   
Sequence Name 15-K104400-0022-835-G07C-BH53
GenBank Accession LN492957 [GenBank]
Sequencing primer gene specific primer, BH53: 5'-CTTCCAAGGACATAACTCGTAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K104400-0022-835-G07C-BH53
AGTTTGAAGCTTTACTCAGATAAATAACCTTTTAACCCATATCTTTCTTGATTGTTTCAG
ACAAGTCAACCAGAACCGACTGGAAACGGTGAAGGCTCAGGCAAAAGAGAGTGTCATCTA
CTCATCTTACATGGTCGAATTCAATTACTTAGTGTGTTTCCTCTGCATTTTGGTTTGGTT
TGGTGAGTTTAGAGAGTGTTTGGTCAGTGTTCATGTCTAGTCTATTACATCATTTTCATA
TCTTGCTTCAATTTACTCTGATATGTTTGAAGACACAAAATAGTTGAAGTAAATAGTTAA
TCTCTGTATAAACCTTTGCAATCACTCCTCTCTAATTACACAGACAAGCCTGCATTTTTA
CATATAGTACACATACAAGCATAAAGCACGATCTGTTTTCTTAAATGTGTTTCATCTTAC
ACGAGGAGATTGAGTGTAAGAAGGTAAAGGCGATGCACCAGGAGTCATGTGCAGCAGAGG
CCGATCATGACTATATCTTACCGGAGATTTCTGCATAACAGAAGAATGATGAAATCTTGG
AGATTCCATTTGGGTGTGTTTGGTTCCAGGTTTAGCAACGGATTCTAGCTGTTCTAATGT
GACTAAAACCTCTGACATTTTTGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 31-K104401-0022-835-G07C-8409
GenBank Accession LN500149 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104401-0022-835-G07C-8409
GCCTTTACAATTGAATATTTCCAAAATGTCAGAGGTTTTAGTCACATTAGAACAGCTAGA
ATCCGTTGCTAAACCTGGAACCAAACACACCCAAATGGAATCTCCAAGATTTCATCATTC
TTCTGTTATGCAGAAATCTCCGGTAAGATATAGTCATGATCGGCCTCTGCTGCACATGAC
TCCTGGTGCATCGCCTTTACCTTCTTACACTCAATCTCCTCGTGTAAGATGAAACACATT
TAAGAAAACAGATCGTGCTTTATGCTTGTATGTGTACTATATGTAAAAATGCAGGCTTGT
CTGTGTAATTAGAGAGGAGTGATTGCAAAGGTTTATACAGAGATTAACTATTTACTTCAA
CTATTTTGTGTCTTCAAACATATCAAAGTAAATTGAAGCAAGATATGAAAATGATGTAAT
AGACTAGACATGAACACTGACCAAACACTCTCTAAACTCACCAAACCAAACCAAAATGCA
GAGGAAACACACTAAGTAATTGAATTCGACCATGTAAGATGAGTAGATGACACTCTCTTT
TGCCTGAGCCTTCACCGTTTCCAGTCGGTTCTGGTTGACTTGTCTGAAACAATCAAGAAA
GATATGGGTTAAAAGGTTATTTATCTGAGTAAAGCTTCAATACTTTTATAAGAATTCTGT
AAATTTGAGTCTTGCTTA

Last Updated on 06.12.2018 16:11