Sequences from confirmation amplicon


Confirmation sequences for line 835H10 and hit At3g62290 are shown.
Line ID 835H10
Locus At3g62290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23052988
Primers used for PCR JU38 - 8474, show primer details
   
Sequence Name 66-K027350-022-835-H10C-JU38
GenBank Accession LN511511 [GenBank]
Sequencing primer gene specific primer, JU38: 5'-CGTATCATGGTTGGATTCCTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K027350-022-835-H10C-JU38
TTGCAGTTTTTTCTTTGGTTTGCATGCGGTGTACTTCTACTTGCTCAAGGGCATTCATCT
TTGATTCATGGTTTGTGTATGATTCATAGAATGAGTGCTTGAATTTTTTAGATCTGTCGT
ATTGACAATACCTGTTCTTGCTAGGTTTCAATGTGGAGACTGTGGAATACAAGAACATTA
GCTTTACCGTGTGGGATGTCGGGGGTCAAGACAAGGTATTGTGATTACAGTCCTTTACCA
GCATTCGACAATTACTATAGCTAAATCCTTGATTCTCCATGAGCTGTCCTATTTTACTGA
ATGGCACTTTCACTATTCCAATATAACCTTTTTTGTTTGCTTGCTCATCCAGATTCGTCC
ATTGTGGAGGCACTACTTCCACAATGAGTATACATGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR JU39 - 8474, show primer details
   
Sequence Name 81-K027351-022-835-H10C-8409
GenBank Accession LN514004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K027351-022-835-H10C-8409
TTTACAATTGAATATATCCTGTGATCAGGGACTTATCTTTGTGGTGGACAGCAATGATCG
TGACCGTGTTGTGGAAGCCAGAGATGAGCTTCACAGAATGTTGAACGAAGTAAAACTCAA
ACAAAAATCTCCTTCTCTAATGTTACATTATGAAGCAGTTGGCATCATTTTGTTTCCTTT
TTGACTGTTGCAGGATGAGTTGAGAGATGCTGTGCTTCTCGTTTTTGCTAACAAGCAAGA
TCTTCCAAATGCGATGAACGCCGCTGAGATAACCGATAAGCTTGGACTTCACTCTCTCCG
TCAACGACACTGGTGAGCCAAAGAAAACCAAACCAAATCTCTTTATTTACCAGATACCTA
TATCGTAACATGAAAAGCTGATGTAAATCGTTGGTGGGGTTTGTTGAACAGGTACATACA
GAGCACATGTGCTACCTCCGGAGAAGGGCTTTATGAGGGACTTGACTGGCTCTCTAACAA
CATCGCAAACAAGGTATACAACTTTACAATTTTTGAAATCGTATTAGTAAGAAATTGATT
GTTAAAGACTTACTCAAATGTGTGATTAAC
   
Sequence Name 82-K027351-022-835-H10C-JU39
GenBank Accession LN514127 [GenBank]
Sequencing primer gene specific primer, JU39: 5'-CTCCTAAGCCTGCAAGTTAATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K027351-022-835-H10C-JU39
ATCATTTCTTACANACGATTTCAAAATTGTAAAGTTGTATACCTTGTTTGCGATGTTGTT
AGAGAGCCAGTCAAGTCCCTCATAAAGCCCTTCTCCGGAGGTAGCACATGTGCTCTGTAT
GTACCTGTTCAACAAACCCCACCAACGATTTACATCAGCTTTTCATGTTACGATATAGGT
ATCTGGTAAATAAAGAGATTTGGTTTGGTTTTCTTTGGCTCACCAGTGTCGTTGACGGAG
AGAGTGAAGTCCAAGCTTATCGGTTATCTCAGCGGCGTTCATCGCATTTGGAAGATCTTG
CTTGTTAGCAAAAACGAGAAGCACAGCATCTCTCAACTCATCCTGCAACAGTCAAAAAGG
AAACAAAATGATGCCAACTGCTTCATAATGTAACATTAGAGAAGGAGATTTTTGTTTGAG
TTTTACTTCGTTCAACATTCTGTGAAGCTCATCTCTGGCTTCCACAACACGGTCACGATC
ATTGCTGTCCACCACAAAGATAAGTCCCTGATCACAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11