Sequences from confirmation amplicon


Confirmation sequences for line 836H05 and hit At1g04630 are shown.
Line ID 836H05
Locus At1g04630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1290586
Primers used for PCR DA43 - 8474, show primer details
   
Sequence Name 15-K107108-0022-836-H05C-8409
GenBank Accession LN492996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K107108-0022-836-H05C-8409
TTACAATTGAATATATTCCCATGGAAACATAGGCTTGTTGAATCACTGTTATGTATTCTG
TTATTAGTATTCCCCGACATTTTGGTTGAAGCTTAATCAGCATGAGTTTAGCCTTAAACG
AATATAAAGTTCGTCAACAATTTGTAAACTCTTAAAGCCACAATTGCAAAGATGTGAGAA
TCTGTTATGATGAAAGCAATTATCTAGTTGCATGTTTTGATAGTAGTTATCGGGATACTG
TTTCTATGTAGATAGATTGTTTTAGTATTATATCTGGCTTGATCATTAACTTCTTATGCA
TTTACAATGGTCCTAAACAAGTCTGATTTGATATCAGTAAGTATCAAGATTAATGCTCTT
ATCAAGATTGATGTGATGTTTAGGGCATTGAAGGAAGAGAAATATGCTGCTCGTAGAACA
ATACTTCCCATTCTCAAAGCAGA
   
Sequence Name 31-K107113-0022-836-H05C-DA43
GenBank Accession LN500186 [GenBank]
Sequencing primer gene specific primer, DA43: 5'-TTCTGCTTGAAGAATGGGAAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K107113-0022-836-H05C-DA43
CTTCTTATGCCCTAAACATCACATCAATCTTGATAAGAGCATTAATCTTGATACTTACTG
ATATCAAATCAGACTTGTTTAGGACCATTGTAAATGCATAAGAAGTTAATGATCAAGCCA
GATATAATACTAAAACAATCTATCTACATAGAAACAGTATCCCGATAACTACTATCAAAA
CATGCAACTAGATAATTGCTTTCATCATAACAGATTCTCACATCTTTGCAATTGTGGCTT
TAAGAGTTTACAAATTGTTGACGAACTTTATATTCGTTTAAGGCTAAACTCATGCTGATT
AAGCTTCAACCAAAATGTCGGGGAATACTAATAACAGAATACATAACAGTGATTCAACAA
GCCTATGTTTCCATGGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11