Sequences from confirmation amplicon


Confirmation sequences for line 837D11 and hit At1g47220 are shown.
Line ID 837D11
Locus At1g47220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17304843
Primers used for PCR BE68 - 8474, show primer details
   
Sequence Name 57-K029918-022-837-D11C-8409
GenBank Accession LN509311 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K029918-022-837-D11C-8409
CCATTTACATGTATATATTTAAACCTGAAAAAGAGAGATTGAGACAAAAGTTCTTACCCA
AGGATGTTGGTTAGGAAGGATGATGAATCGAGCGAGAAAGACAGCAGAAGCAGCCAACAA
AGATGGAACAAACTTCACACAACTATAATCTAACATACTTAGTTCTGACAGATAGCAACA
AAGAGGCTCTAGCTGCAAGTTTGGCACTTTGAAATCTTCTTGTGCAACCCTTATGAACCG
TCTGCACAAACACAACAAAAGATGAGAACAAAGAACCATAAGATACAACCAAAAGTCAGT
ATTATTCATCACAATTCTAACCTTAGAAAAGTATTTGTCGTAGGCCTTCCTAGTTCAAAC
TCAAGCGCGAGAAGTATATCCTCTTCCATCTTCAACACATCTTGTTTCGTATAAGTATTA
GCCGTAATGTAACAAAAATCCTCCACTTTAGGGCGACGTTTCTCTTCATACTTCCTATTT
ATATCAAATGCCAAAAAAGAGAAGCCACTCCATATGTAAGTTTCAACATGACAATGAATA
GCAAAGAGAGTAATACAAGAGGATCAAA
   
Sequence Name 57-K029918-022-837-D11C-BE68
GenBank Accession LN509312 [GenBank]
Sequencing primer gene specific primer, BE68: 5'-GCTTCGTAAGTGTTTTTGATCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K029918-022-837-D11C-BE68
GTCATGTTGAACTTACATATGGAGTGGCTTCTCTTTTTTGGCATTTGATATAAATAGGAA
GTATGAAGAGAAACGTCGCCCTAAAGTGGAGGATTTTTGTTACATTACGGCTAATACTTA
TACGAAACAAGATGTGTTGAAGATGGAAGAGGATATACTTCTCGCGCTTGAGTTTGAACT
AGGAAGGCCTACGACAAATACTTTTCTAAGGTTAGAATTGTGATGAATAATACTGACTTT
TGGTTGTATCTTATGGTTCTTTGTTCTCATCTTTTGTTGTGTTTGTGCAGACGGTTCATA
AGGGTTGCACAAGAAGATTTCAAAGTGCCAAACTTGCAGCTAGAGCCTCTTTGTTGCTAT
CTGTCAGAACTAAGTATGTTAGATTATAGTTGTGTGAAGTTTGTTCCATCTTTGTTGGCT
GCTTCTGCTGTCTTTCTCGCTCGATTCATCATCCTTCCTAACCAACATCCTTGGGTAAGA
ACTTTTGTCTCAATCTCTCTTTTTCAGGTTTAAATATATACATGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11