Sequences from confirmation amplicon


Confirmation sequences for line 838E03 and hit At5g17260 are shown.
Line ID 838E03
Locus At5g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5676600
Primers used for PCR 56kh - 8474, show primer details
   
Sequence Name 80-K113595-0022-838-E03C-56kh
GenBank Accession LN513972 [GenBank]
Sequencing primer gene specific primer, 56kh: 5'-TGATTCCTAGCAAAGACCAAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113595-0022-838-E03C-56kh
GGTTCAGGACAACCGGGCAAAAAAGGCGGTTATTGGAAAGCCACAGGCAAAGACCGGCGG
GTGAGTTGGAGAGATCGAGCCATTGGGACCAAGAAAACATTAGTTTACTACCGTGGACGT
GCCCCGCATGGTATTAGAACTGGTTGGGTCATGCATGAATATCGCCTCGATGAATCCGAA
TGCGAGCCTTCTGCATTTGGCATGCAGGTTTACTTACTAACCAAACCAATCATCAATCAT
CTACTTTAAAGTAGTAGATGTATTACTAGGGAAAACTATATTCTAACAAGATTCTTGTTC
TATTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 38-K113594-0022-838-E03C-8409
GenBank Accession LN503052 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113594-0022-838-E03C-8409
TCATTGAATATATCCTGAAATAGAACAAGAATCTTGTTAGAATATAGTTTTCCCTAGTAA
TACATCTACTACTTTAAAGTAGATGATTGATGATTGGTTTGGTTAGTAAGTAAACCTGCA
TGCCAAATGCAGAAGGCTCGCATTCGGATTCATCGAGGCGATATTCATGCATGACCCAAC
CAGTTCTAATACCATGCGGGGCACGTCCACGGTAGTAAACTAATGTTTTCTTGGTCCCAA
TGGCTCGATCTCTCCAACTCACCCGCCGGTCTTTGCCTGTGGCTTTCCAATAACCGCCTT
TTGTTGCCCGGTTTGTCCTTGAACCATTGGGGTACTTTCTGTCCCGTGGGCTGAAGAAGA
ACCATTCTTGGTCTTTGCTAGGAATC

Last Updated on 06.12.2018 16:11