Sequences from confirmation amplicon


Confirmation sequences for line 838F01 and hit At1g22340 are shown.
Line ID 838F01
Locus At1g22340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7892345
Primers used for PCR MG37 - 8474, show primer details
   
Sequence Name 73-K032738-022-838-F01C-MG37
GenBank Accession LN512829 [GenBank]
Sequencing primer gene specific primer, MG37: 5'-TTGACATGTAACTTTCGTCTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K032738-022-838-F01C-MG37
TATCTAAATTAAGTATACACATATTACAACTTTGGGCTAAAGACAGATAAATATGCTAGA
TGCATTAGTTACGGACGTTTTTTCTTTTAACTATATGAATTAAAATCAGTAGTAATAGAA
AAACAATTAGTACCTTTAAAAGGAGATAGACCCTTCTCGATGAAAAGATAAAAGTGTAGA
ATAGTCATGAAACCACAAGCACTATTGGTCCAAAAAATAACCTCTGGGACACCTAGTTCC
TCGGCTGCGTCAAGAGTAAAACTCATCACACCGTCCGATACAATACAACTCACTGGAGGA
ACATCATCTTTATCGTTGATCCGGCGCAGAATCTCTTTGAATGGAGCGAGACAGTTTTTC
TCAATGGACATGCAAACGGTAGGAGTATGCTGCGTCCTATCGCCATCAGTCTCCGGTAGA
CCGTCAGGGATGGACTCGAACCGGAATGAAGGAAACCCGTCGAGCGCGTTGGGACCACGG
GACCGGAGGAGACGGTTGTGGTTGTAGAGAGTGTTAACGAAGGTGACGTGAAAGCCTTTA
GCGTAGAGGAGTTTAGCCACTTTCAGCATCGGATTGATGTGGCCTTGAGCCGGGTAAGGC
ACGCAAACTACGTGTGGCTTTTGTGCGTTATGAACAACATGAGATTCCATTTTCTTGCTC
TCTTATGCCGAGAAAAAATGAAAATGGATTTGTATATGTGATTATAAGAACTTCGTTTTA
GAAAGATAATTGACAATGAGTTGGTGAGACCTTATGATTCTAGCACGTGGGAATTAACAA
AAGATATGTGTCAGCGTCACATGCGAAGATCGAGGCCGAACTTTTGTATCTATGTGTGAA
ATTTATAAGCCTAGCAAAAGATAGTATTGGTTTATCTAAAGATTGAGTTTTTTTACGAAG
GAGATACACTAATGGCTA
   
Sequence Name 41-K032738-022-838-F01C-8409
GenBank Accession LN503976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K032738-022-838-F01C-8409
CGGAATGAGCCTTTATTAGCCATTAGTGTATCTCCTTCCGTAAAAAACTCAATCTTTAGA
TAAACCAATACTATCTTTTGCTAAGCTTATAAATTTCACACATAGATACAAAAGTTCGGC
CTCGATCTTCGCATGTGACGCTGACACATATCTTTTGTTAATTCCCACGTGCTAGAATCA
TAAGGTCTCACCAACTCATTGTCAATTATCTTTCTAAAACGAAGTTCTTATAATCACATA
TACAAATCCATTTTCATTTTTTCTCGGCATAAGAGAGCAAGAAAATGGAATCTCATGTTG
TTCATAACGCACAAAAGCCACACGTAGTTTGCGTGCCTTACCCGGCTCAAGGCCACATCA
ATCCGATGCTGAAAGTGGCTAAACTCCTCTACGCTAAAGGCTTTCACGTCACCTTCGTTA
ACACTCTCTACAACCACAACCGTCTCCTCCGGTCCCGTGGTCCCAACGCGCTCGACGGGT
TTCCTTCATTCCGGTTCGAGTCCATCCCTGACGGTCTACCGGAGACTGATGGCGATAGGA
CGCAGCATACTCCTACCGTTTGCATGTCCATTGAGAAAAACTGTCTCGCTCCATTCAAAG
AGATTCTGCGCCGGATCAACGATAAAGATGATGTTCCTCCAGTGAGTTGTATTGTATCGG
ACGGTGTGATGAGTTTTACTCTTGACGCAGCCGAGGAACTAGGTGTCCCAGAGGTTATTT
TTTGGACCAATAGTGCTTGTGGTTTCATGACTATNCTACACTTTTATCTTTTCATCGAGA
AGGGTCTATCTCCTTTTAAAGGTACTAATTGTTTTTCTATTACTACTGATTTTAATTCAT
ATAGTTAAAAGAAAAAACGTCCGTAACTAATGCATCTAGCATATTTATCTG

Last Updated on 06.12.2018 16:11