Sequences from confirmation amplicon


Confirmation sequences for line 839A12 and hit At1g02130 are shown.
Line ID 839A12
Locus At1g02130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:400680
Primers used for PCR KU86 - 8474, show primer details
   
Sequence Name 14-K103353-0022-839-A12C-KU86
GenBank Accession LN492470 [GenBank]
Sequencing primer gene specific primer, KU86: 5'-TTACTGAAAACAGAGCCATTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103353-0022-839-A12C-KU86
ATTCTTTCTTCTCAGTTTTGAAATCATCAAACTCTAAGGCTATTATTACTTTCCTGATTA
CTTCGAAACATTCATAGGCTTTTGCCGATGAAATCGGGATTCCTTTTATGGAGACTAGTG
CAAAAGATGCTACAAACGTAGAACAGGCTTTCATGGCAATGTCTGCATCCATCAAAGAGA
GGTATTTATCTTACATCGTTCTTTTTAAGTAACTGCGGTTAATATAGAGCTATTACAAAA
ATGAGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 30-K103354-0022-839-A12C-8409
GenBank Accession LN499679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103354-0022-839-A12C-8409
ATTTACAATTGAATATATCCTGTCCTCATTTTTGTAATAGCTCTATATTAACCGCAGTTA
CTTAAAAAGAACGATGTAAGATAAATACCTCTCTTTGATGGATGCAGACATTGCCATGAA
AGCCTGTTCTACGTTTGTAGCATCTTTTGCACTAGTCTCCATAAAAGGAATCCCGATTTC
ATCGGCAAAAGCCTATGAATGTTTCGAAGTAATCAGGAAAGTAATAATAGCCTTAGAGTT
TGATGATTTTCAAAACTTGAGAAGAAAGAATTGGTTCAAGTGGCATATATATACCTTGGC
AGTTTCATAAGGAATGGCTCTGTTTTCAGTA

Last Updated on 06.12.2018 16:11