Sequences from confirmation amplicon


Confirmation sequences for line 839C02 and hit At1g19940 are shown.
Line ID 839C02
Locus At1g19940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6919542
Primers used for PCR NS48 - 8474, show primer details
   
Sequence Name 29-K102500-0022-839-C02C-8409
GenBank Accession LN499232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K102500-0022-839-C02C-8409
TTACATTGAATAATCCTGCAGAGACAGCAGCAGCTATGGCTGCAGCATCTTTAGTCTTTA
AAGAAAGTGATACCAAGTATTCAAGCACGCTTTTGAAACATGCGAAGCAGTTGTTCGATT
TTGCGGATAATAACAGAGGATCTTACAGTGTTAATATACCTGAGGTTCAGTCTTATTATA
ACTCAACTGGTTATGGAGATGAGCTTCTTTGGGCAGCTTCATGGTTGTACCATGCAACAG
AGGATCAAACATATCTTGACTTTGTGTCGGAAAATGGTGAAGAGTTTGGTAATTTTGGAA
GTCCATCATGGTACNNCCNTGNNGCTGCCCNNNGAAGCTCTTCTCCATNACCAGTTGAGT
   
Sequence Name 29-K102501-0022-839-C02C-NS48
GenBank Accession LN499233 [GenBank]
Sequencing primer gene specific primer, NS48: 5'-ACCATGATGGACTTCCAAAATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K102501-0022-839-C02C-NS48
GATATGTTTGATCCTCTGTTGCATGGTACAACCATGAAGCTGCCCAAAGAAGCTCATCTC
CATAACCAGTTGAGTTATAATAAGACTGAACCTCAGGTATATTAACACTGTAAGATCCTC
TGTTATTATCCGCAAAATCGAACAACTGCTTCGCATGTTTCAAAAGCGTGCTTGAATACT
TGGTATCACTTTCTTTAAAGACTAAAGATGCTGCAGCCATAGCTGCTGCTGTCTCTGCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11