Sequences from confirmation amplicon


Confirmation sequences for line 839D07 and hit At1g75860 are shown.
Line ID 839D07
Locus At1g75860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28485440
Primers used for PCR 22hj - 8474, show primer details
   
Sequence Name 47-K112479-0022-839-D07C-22hj
GenBank Accession LN506238 [GenBank]
Sequencing primer gene specific primer, 22hj: 5'-AATCGAATGTTTATGCGATTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K112479-0022-839-D07C-22hj
TCTTCTTTCCTTCTTCTCTATCCGGTGCGCCTTTTTTGAATCAGTGTGATTCTTTTAAGC
TTTAATCCATTAGAGTAAAGGGTTTTAGACTCCACGTCTTTCAGAACCACAGTTCAACCA
TCCCACTCAATGTGCAAAAACCATTACAATAAACAAAGAAGCTCCTACAAACTTAATCAT
ATACATATCTAGACCTACAAAATCAAACTAACAGAATGGAAAACAATGCAACTTACCTGA
TTCACTCCTAATGATTGCTGATACAGTGAACACATGATGAGATTATTGAAAACTAATATG
CAACTCTTTATATAGTGTTAATGATAATCACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 17-K112478-0022-839-D07C-8409
GenBank Accession LN493916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112478-0022-839-D07C-8409
ATATATCCTGTGATTATCATTAACACTATATAAAGAGTTGCATATTAGTTTTCAATAATC
TCATCATGTGTTCACTGTATCAGCAATCATTAGGAGTGAATCAGGTAAGTTGCATTGTTT
TCCATTCTGTTAGTTTGATTTTGTAGGTCTAGATATGTATATGATTAAGTTTGTAGGAGC
TTCTTTGTTTATTGTAATGGTTTTTGCACATTGAGTGGGATGGTTGAACTGTGGTTCTGA
AAGACGTGGAGTCTAAAACCCTTTACTCTAATGGATTAAAGCTTAAAAGAATCACACTTG
ATTCAAAAAAGGCGCACCGGATAGAGAAGAAGGAAAAGAAGGAGAAGAGAAAAGAAAAGA
AAGAAACTAAGCGTGAGAAATCGCATAAACATTCGAT

Last Updated on 06.12.2018 16:11