Sequences from confirmation amplicon


Confirmation sequences for line 839H05 and hit At2g33110 are shown.
Line ID 839H05
Locus At2g33110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14042582
Primers used for PCR MN25 - 8474, show primer details
   
Sequence Name 50-K116518-0022-839-H05C-MN25
GenBank Accession LN507292 [GenBank]
Sequencing primer gene specific primer, MN25: 5'-TGTCTTGGTCAGTGGTTTTACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116518-0022-839-H05C-MN25
CGTCAGTTTTCTAGATCTCTAAGCTTTCGTGCTCGCCGATATAAATCTCTAATATCGGTC
CCGTAAGATCGAATTAGATTCGCGTCGTTTTTTAAGGAACCAAAATAATGGCGCAACAAT
CGTTGTTCTACAGTTTCATCGCTCGCGGCACCGTAATCCTCGTCGAGTTCACAGATTTCA
AAGGCAATTTCACATCTGTCGCTGCTCAGTACCTTGAGAATCTTCCTTCCTCGAACAACA
AGTTTACCTACAACTGCGATGGTCATACGTTCAACGACCTCGTCGAAAATGGATTCAGTG
AGTCAAAATATTGCTCGTGATGTGTTTGTGATTGTGTTTTCGATTAGTCGATTTATTCAT
TGTTTGGATTAGATTTCTTTTTACCTAATTGAGCATTTGAGAATCGAGTTCTTATGTTTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 24-K116517-0022-839-H05C-8409
GenBank Accession LN497145 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116517-0022-839-H05C-8409
GCCATTTCAACATAAGAACTCGATTCTCAAATGCTCAATTAGGTAAAAAGAAATCTAATC
CAAACAATGAATAAATCGACTAATCGAAAACACAATCACAAACACATCACGAGCAATATT
TTGACTCACTGAATCCATTTTCGACGAGGTCGTTGAACGTATGACCATCGCAGTTGTAGG
TAAACTTGTTGTTCGAGGAAGGAAGATTCTCAAGGTACTGAGCAGCGACAGATGTGAAAT
TGCCTTTGAAATCTGTGAACTCGACGAGGATTACGGTGCCGCGAGCGATGAAACTGTAGA
ACAACGATTGTTGCGCCATTATTTTGGTTCCTTAAAAAACGACGCGAATCTAATTCGATC
TTACGGGACCGATATTAGAGATTTATATCGGCGAGCACGAAAGCTTTAGAGATCTAGAAA
ACTTGACGCTGAAACGCGTAGAGAATCGAAGACTAAGCCAAGTAAAACCACTGACCAAGA
C

Last Updated on 06.12.2018 16:11