Sequences from confirmation amplicon


Confirmation sequences for line 840D06 and hit At5g46580 are shown.
Line ID 840D06
Locus At5g46580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18899097
Primers used for PCR FQ62 - 8474, show primer details
   
Sequence Name 45-K034241-022-840-D06C-8409
GenBank Accession LN505443 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K034241-022-840-D06C-8409
AAGCCATTTACAATTGAAACTATATTTTACAATGTTACAATGAAGTCTCTGAGATTTGGG
AGGCAATTTCAGCTTATTGAGGAGATGGCACTTGAAATGGTGAAGGATGGTGTTGAGCTT
GATAACATTACTTATTCGACAATCATCACTTGTGCTAAAAGGTGCAATCTTTACAACAAA
GCTATTGAGTGGTTTGAGAGAATGTATAAAACAGGTCTGATGCCTGATGAGGTCACTTAC
TCAGCAATTCTTGATGTTTATTCCAAGTCAGGAAAAGTTGAGGAGGTTCTTAGTTTGTAC
GAAAGAGCGGTGGCTACGGGTTGGAAACCTGATGCTATAGCGTTCTCTGTGTTGGGGAAA
ATGTTTGGTGAAGCTGGAGATTATGATGGGATTAGGTAGNTTTTGCAAGAAAATTGAGTA
CTTACCATTTTTTTTTCATTTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GAACATTTTGATAAATCGACAAATTACGATCCGTCATACTTATAAGCATAAAGCAATAAA
CAAATTATTCAAATTCTGAAATCATTA
   
Sequence Name 46-K034241-022-840-D06C-FQ62
GenBank Accession LN505788 [GenBank]
Sequencing primer gene specific primer, FQ62: 5'-CTTGCAAAACATACCTAATCCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K034241-022-840-D06C-FQ62
CATTTTTCCCCACACAGAGAACGCTATAGCATCAGGTTTCCAACCCGTAGCCACCGCTCT
TTCGTACAAACTAAGAACCTCCTCAACTTTTCCTGACTTGGAATAAACATCAAGAATTGC
TGAGTAAGTGACCTCATCAGGCATCAGACCTGTTTTATACATTCTCTCAAACCACTCAAT
AGCTTTGTTGTAAAGATTGCACCTTTTAGCACAAGTGATGATTGTCGAATAAGTAATGTT
ATCAAGCTCAACACCATCCTTCACCATTTCAAGTGCCATCTCCTCAATAAGCTGAAATTG
CCTCCCAAATCTCAGAGACTTCATTGTAACATTGTAAAATATAGTTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR CB47 - 3144, show primer details
   
Sequence Name 16-K104847-0022-840-D06C-3144
GenBank Accession LN493422 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K104847-0022-840-D06C-3144
CCTTGCAGACCCTTCCTCTGTATAGGAAGTTCATTTCATTTGCAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGGAAACAGACT
CTTGGATTTAACCCAATTAAAGAAAGTATGAGTCTTTTGCCATTCTCTTAAGCTGTTTAG
CACAAGCAATGCGTTATCTCTATTGGGTGGATGAGGAATCTCATCGAGAAGAGATAAAAA
CTCAGACTTTTCAGTGAAAATAGAACTATTGAGCTTCAGAGCAAAAGCCCTAAGATCCTT
GATTTGAGGATTGTAAGAGTAAGCAGAACGCTTTTGTCTCTGAAGTGAAAGAACTGACCT
TTTGGGTCTTGTTGGATTGACCCAGACAGATTTCGGTTTAGACAAGATTTGAGTTTGCTC
TTGTCTGAGTGTTGAAAC
   
Sequence Name 01-K104846-0022-840-D06C-CB47
GenBank Accession LN486566 [GenBank]
Sequencing primer gene specific primer, CB47: 5'-CAACACTCAGACAAGAGCAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104846-0022-840-D06C-CB47
GAGACCCAAAGGTCAGTTCTTGTCCTTCAGAGACAACCGTGATCGGCTTACTCTTACAAT
CCTCAAATCAAGGATCTTAGGGCTTTTGCTCTGAAGCTCAATAGTTCTATTTTCACTGAA
AAGTCTGAGTTTTTATCTCTTCTCGATGAGATTCCTCATCCACCCAATAGAGATAACGCA
TTGCTTGTGCTAAACAGCTTAAGAGAATGGCAAAAGACTCATACTTTCTTTAATTGGGTT
AAATCCAAGAGTCTGTTTCCTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAA
TCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGG
GTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCA
ATCCA

Last Updated on 06.12.2018 16:11