Sequences from confirmation amplicon


Confirmation sequences for line 840E05 and hit At2g25010 are shown.
Line ID 840E05
Locus At2g25010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10632740
Primers used for PCR NS47 - 8474, show primer details
   
Sequence Name 68-K102596-0022-840-E05C-8409
GenBank Accession LN511984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K102596-0022-840-E05C-8409
CCGGAATGAGCATTTACATTGAATATCATAAGGATCAAGATCGTCCAGTTCTCCGTTGTA
CTCAGATAGGTCGGTTTTGGATCTGCTTCCTTTACCTTTCCACAAAAGAGCAAGCGGGAA
ACACGCTTCGGATTTCTCGGGTCTACCGATATCCAGATGAAAATAACTCCAACACTATAT
ACATGAGACAATCATATGTTACATTTCCTAAAGATATTGAAATCTCAACTGGACAGAAAA
CCAAAACTAACATAGAAGCCAACCTGTAGCAAAGTCAAACAACCGCAAATGTTGCTCTGA
GATTTAAGCGACGCATTACCGAGCGCTCGGTAAAGACAAGCCAACGCAGCAGCTCCCCAA
GCATATCTCCCAGCTTGATCAAAATCCTCAAACAAAGGCAAATACTTAACAGAGACTTTA
TCACCATCAGTAGTAGCAAAAATGGTACTACCAATAAGATACAAAAGATAAGCTCGAGTA
TGACATTTAACAACATCGAAAGAAGC
   
Sequence Name 68-K102597-0022-840-E05C-NS47
GenBank Accession LN511985 [GenBank]
Sequencing primer gene specific primer, NS47: 5'-TGCTTCTTTCGATGTTGTTAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K102597-0022-840-E05C-NS47
CATTTTTGCTACTACTGATGGTGATAAAGTCTCTGTTAAGTATTTGCCTTTGTTTGAGGA
TTTTGATCAAGCTGGGAGATATGCTTGGGGAGCTGCTGCGTTGGCTTGTCTTTACCGAGC
GCTCGGTAATGCGTCGCTTAAATCTCAGAGCAACATTTGCGGTTGTTTGACTTTGCTACA
GGTTGGCTTCTATGTTAGTTTTGGTTTTCTGTCCAGTTGAGATTTCAATATCTTTAGGAA
ATGTAACATATGATTGTCTCATGTATATAGTGTTGGAGTTATTTTCATCTGGATATCGGT
AGACCCGAGAAATCCGAAGCGTGTTTCCCGCTTGCTCTTTTGTGGAAAGGTAAAGGAAGC
AGATCCAAAACCGACCTATCTGAGTACCGCAGAGAACTGGACGATCTTGATCCTTATGAA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11