Sequences from confirmation amplicon


Confirmation sequences for line 840G03 and hit At2g41670 are shown.
Line ID 840G03
Locus At2g41670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17374759
Primers used for PCR JF29 - 8474, show primer details
   
Sequence Name 40-K102175-0022-840-G03C-8409
GenBank Accession LN503660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K102175-0022-840-G03C-8409
TTACAATTGAATATATCCTGCGCATTGAAAAGTTACCTTCTTTGCATAATTTTTCGTATG
TAGCTGCTTGATCTCGTGGAGTTGAAGCTCAAAGAAGTGATTGCTAGGGAGCCTACTTTA
CTTGTAATGGTGGTTGGTGTCCCCAATGTTGGAAAATCTGCGCTCATTAACTCGATCCAT
CAAATTGCAGCAGCTCGTTTTCCGGGTAAGATATTGGTGTCTCAGCTTATCAGCAGTGTT
GAGTACACATTTCACAGATATAGTCCGGACTGTGAATCCGATTTCTCCAAACTTAAACTT
ACAAGTTAATTATTTCATGAACAGAATGATGAATGAAATGTAACGTGGCTTATTTCGTTA
TTGTATGCATTATGTGCATAGTGCAGGAGAGATTAAAGAGAGCTACGGTTGGTCCATTGC
CTGGTGTAACACAAGATATTGCTGGTTTCAAGGTAATCTTTATTTGAAAATCCTCTTTTT
TCCGTATGCCTGTTAATTGTTTGTGGATTTGCTTCAGCATGATTTTTGAACACATGATGA
AAATGTTCTGTGGTACAGATTGCTCATCGACCTAGTATATATGTTCTAGACTCCCCTGGT
GTGTTGGTTCCAAGTATCC
   
Sequence Name 40-K102179-0022-840-G03C-JF29
GenBank Accession LN503661 [GenBank]
Sequencing primer gene specific primer, JF29: 5'-GTTTCGATATCAGGGATACTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K102179-0022-840-G03C-JF29
TCGATGAGCAATCTGTACCACAGAACATTTTCATCATGTGTTCAAAAATCATGCTGAAGC
AAATCCACAAACAATTAACAGGCATACGGAAAAAAGAGGATTTTCAAATAAAGATTACCT
TGAAACCAGCAATATCTTGTGTTACACCAGGCAATGGACCAACCGTAGCTCTCTTTAATC
TCTCCTGCACTATGCACATAATGCATACAATAACGAAATAAGCCACGTTACATTTCATTC
ATCATTCTGTTCATGAAATAATTAACTTGTAAGTTTAAGTTTGGAGAAATCGGATTCACA
GTCCGGACTATATCTGTGAAATGTGTACTCAACACTGCTGATAAGCTGAGACACCAATAT
CTTACCCGGAAAACGAGCTGCTGCAATTTGATGGATCGAGTTAATGAGCGCAGATTTTCC
AACATTGGGGACACCAACCACCATTACAAGTAAAGTAGGCTCCCTAGCAATCACTTCTTT
GAGCTTCAACTCCACGAGATCAAGCAGCTACATACGAAAAATTATGCAAAGAAGGTAACT
TTTCAATGCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATG

Last Updated on 06.12.2018 16:11