Sequences from confirmation amplicon


Confirmation sequences for line 841D12 and hit At1g49620 are shown.
Line ID 841D12
Locus At1g49620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18366262
Primers used for PCR BG33 - 8474, show primer details
   
Sequence Name 23-K101744-0022-841-D12C-8409
GenBank Accession LN496495 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101744-0022-841-D12C-8409
TTACATTGAAATGAGTATAATCGGATAAGATTACAAATTTTGAAATTTCTCTGTTTCACA
ATAAATGCTCTCTGAGCAAGATCTGATTACGTTTTTTTGGATTAATTAATTTTGTTTCCG
TTTTCAGGCTCATGAAATCTCCGAAACCGAAATCTCAACGTTACTCACCAACAATTTCAG
GTTAATTACTAAAAAAGACGTTTCAAAGATTTTTCAATTTCTTCATTGTTTCGTCTATAA
ATTCATAATTTTTGGAATTTCACAGGAAACAGGGAATTTCATCAAGCGAGAATCTGGGAG
AAACAGCAGAAATGGACTCGGCGACGACGGAGATGAGAGATCAGAGAAAGACGGAGAAGA
AGAAGAAGATGGAAAAATCACCGACGCAGGCAGAGCTTGATGACTTTTTCTCGGCGGCGG
AGAGATACGAACAGAAACGATTCACAGAAAAGTGAGTCTTTTTTAGACCCACACACACGC
GCATGATAGCAGAGATCCTTAACTTGCAGTTGTTTAAAACATGTATAGGTACAACTACAA
CATCGTCAATGATACGCCGCTNGAAGGTCGGTACCAGTGGGTTAGTCTGAAACCTTAGAA
GCCATGGANGAACNNAANAAAAANGAAAAAGAAGAAGAGACGGTGCTAAAGTTACACGTC
CAAGTACTGTGATTTTTGTCACAAACAGAAAAATTTTTAGAGACTTTTCATTGTACAGTT
TTAATCTAATTTTAATTTCATCATTATCTTCATCTGTTCTTCGAACCAAGAGTTTTTTTT
TT
   
Sequence Name 23-K101750-0022-841-D12C-BG33
GenBank Accession LN496496 [GenBank]
Sequencing primer gene specific primer, BG33: 5'-GCACAACACCAAATTCACTACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101750-0022-841-D12C-BG33
TTTCTGACACATAATTCCAATTTTTAATTGTTGTAACCCCACTTTTTTTGTTGAACAATC
CAACAATATCACCGAGACAGAGAGAATTAAACTCAAATATATGTTAACACTTCTAGTTAA
AAAAAAAAACTCTTGGTACGAAGAACAGATGAAGATAATGATGAAATTAAAATTAGATAA
AAACTGTACAATGAAATGTCTCTAAAAATTCTTCTGTCTGTGACAAAAATCACAGTACTA
GGACGTGTAACTTTAGCACTGTCTCTTCTTCTTTTTCTTTTTTTTTTTGTTCTCCCAGGG
CTTCAAAGGTTTCAAACTAACCCACGGGTACCGACTTTCAAGCGGCGTACCATTGACAAT
TTCGAATTTGTACCTATACTGGTTTTAAACAACGGCAATTTAAGGATCTCTGCTATCAGG
CGCGGGGGTGGGGGTCTAAAAAAAACCCATTTTTCGGGGAACCGTTTCGGTTCGAATCTC
CCCCCCCCCAAAAAAAATTCATCAAGCTCTGCCGGCGTCGGTAATTTTTCCATCTTCTTC
TTCTTCCCCGTCTTTCTTGGATCTCTAATCCCCGTCGTCCCCAAGTCCATTTCTGCTGTT
TCTCCCAAATTCTCGCTGGAGAAATTTCCCGGTTTCCGGTGAAATTCCAAAAATTATGAA
TTTTTGGACAAAACATTGAAAAATTTGAAAAATCTTTGAAACGTCTTTTTTAGTAATTAA
CCGGAAATTGTTGGTGAGTAACGTTAAAATTTCGGTTTCGG

Last Updated on 06.12.2018 16:11