Sequences from confirmation amplicon


Confirmation sequences for line 841E08 and hit At1g43890 are shown.
Line ID 841E08
Locus At1g43890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16648332
Primers used for PCR 16cc - 8474, show primer details
   
Sequence Name 55-K108225-0022-841-E08C-16cc
GenBank Accession LN508836 [GenBank]
Sequencing primer gene specific primer, 16cc: 5'-TTGTCGGGAATAAAGTTGATAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K108225-0022-841-E08C-16cc
TTGAGTGTCTATATGTTGCATAGCAATTGATCTTTATGTAATTTCCAGGAGAGTGAAAGA
GCTGTTTCTAAAAAAGAAGGCATAGACTTTGCTCGGGAGTACGGATGTTTGTTTCTGGAG
TGTAGTGCAAAGACTCGAGTCAACGTTGAGCAATGTTTTGAAGAGCTTGTTCTTAAGGTA
GTAAATTTTGCAGCTAGCTTCTAGATTTTTGTTCTGAGGATATATGAGTATGTCGTAATC
TTTCTTTATTTGCAGATATTGGAAACGCCGAGTCTTACTGCTGAAGGTTCGTCTGGCGGA
AAGAAGAACATCTTCAAACAAAACTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTA
   
Sequence Name 39-K108224-0022-841-E08C-8409
GenBank Accession LN503379 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K108224-0022-841-E08C-8409
GAGAGTTCTTCTTTCCGCCGACGAACCTTCAGCAGTAAGACTCGGCGTTTCCAATATCTG
CAAATAAAGAAAGATTACGACATACTCATATATCCTCAGAACAAAAATCTAGAAGCTAGC
TGCAAAATTTACTACCTTAAGAACAAGCTCTTCAAAACATTGCTCAACGTTGACTCGAGT
CTTTGCACTACACTCCAGAAACAAACATCCGTACTCCCGAGCAAAGTCTATGCCTTCTTT
TTTAGAAACAGCTCTTTCACTCTCCTGGAAATTACATAAAGATCAATTGCTATGCAACAT
TATAGACACTCAACATGAGGACGGAGAGACGAGATGATCTTACCTTATCAACTTTATTCC
CGACA

Last Updated on 06.12.2018 16:11