Sequences from confirmation amplicon


Confirmation sequences for line 843F10 and hit At2g27860 are shown.
Line ID 843F10
Locus At2g27860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11866149
Primers used for PCR 46uv - 8474, show primer details
   
Sequence Name 48-K117939-0022-843-F10C-46uv
GenBank Accession LN506632 [GenBank]
Sequencing primer gene specific primer, 46uv: 5'-GAGACTTGAAGGTCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117939-0022-843-F10C-46uv
CGATTCGTTTTTTGATCTCTTGTTCTTCGGTGTTATGATAGAAGTCTCAGATCTGATTCG
TTATCGGAATTCAGATGTTTCGCAATGTATTGAGATCGGTGATGTTGTGATGTAATTCTG
ATGTTTCTTTTTGGATTCGCAGATTATAAATCTTGCTGCGATCTGTACTCCAGCTGATTA
CAATACACGTCCTCTTGATACTATCTACAGCAATTTCATTGATGCGCTTCCAGTTGTATG
TGTTTTCTTCTTAGCTTCGTTCATTGTTTTGCTTTAGCTCTTGTTTTTGAAGTTGATGGA
GACAGTGATGATTGTGTAATGCGTTGGTTTTGTTAGGTGAAATACTGTTCTGAGAACAAC
AAGCGTCTCATTCACTTTTCTACCTGTGAAGTTTATGGAAAAACCATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 39-K117938-0022-843-F10C-8409
GenBank Accession LN503506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117938-0022-843-F10C-8409
TTTTTCATAAACTTCACAGGTAGAAAAGTGAATGAGACCCTTGTTGTTCTCAGAACAGTA
TTTCACCTAACAAAACCAACGCATTACACAATCATCACTGTCTCCATCAACTTCAAAAAC
AAGAGCTAAAGCAAAACAATGAACGAAGCTAAGAAGAAAACACATACAACTGGAAGCGCA
TCAATGAAATTGCTGTAGATAGTATCAAGAGGACGTGTATTGTAATCAGCTGGAGTACAG
ATCGCAGCAAGATTTATAATCTGCGAATCCGAAAAGAAACATCAGAATTACATCACAACA
TCACCGATCTCAATACATTGCGAAACATCTGAATTCCGATAACGAATCAGATCTGAGACT
TCTATCATAAACACCGAAGAACAAGAGATCAAAAAACGAAATCGATAAGCATTTTTACCA
GATCCGCCATCTTAACAAGACCTTCAAGTCT

Last Updated on 06.12.2018 16:11