Sequences from confirmation amplicon


Confirmation sequences for line 843H01 and hit At4g39740 are shown.
Line ID 843H01
Locus At4g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18436814
Primers used for PCR AU21 - 8474, show primer details
   
Sequence Name 41-K106288-0022-843-H01C-8409
GenBank Accession LN504088 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K106288-0022-843-H01C-8409
ATTTACTGGACACGCTAAGATTCATATAATGTTTTAAGATCAAGAATTTCGATACTTTAG
AAGATAAACCAAACAAGAAACATTCACAAGACCATCAAAATTCCAACAAAGAGAGACTTT
TTTCGCAGATAGAGTAACAATTGTTCTACTTAAAAAACCCAGATAAAGTAAAATCCCCTA
AGCAACTTAAAATTAGGGTTTATAAGGGGTTTAAGGATAAGAAGACTAACCTTCGGAGAA
AACTGGCAGCTTGATTCTTGCAGGACAGAACAAGACGGCGACAAGGAAGCATTGAATTTG
GTTCTTCTTCTCTGTTTATTTGATAAATTATTTCAGTTTCATCCAAAAGAAAACTTATTC
CGTACTATAGAATCTTTTTTTCTTGGTGAAGAGCCAAAACCAAAACAAAATAAAAAAAGT
TTTTGTTTTTTTCTTGGTGGCCAAGCTCATTTCCGTGTGTTTTAATGTTTTTATTTGTTT
AATTTTGTGTAAAGTAATTAAATTATCTATGGGTTGACTTACTAATAAATTATTGGGCTT
CATAATTGACACACAAAAAATTATTGAGCTTTATATACAACAATTTGA
   
Sequence Name 41-K106289-0022-843-H01C-AU21
GenBank Accession LN504089 [GenBank]
Sequencing primer gene specific primer, AU21: 5'-GCCCAATAAAACTCAAATTGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K106289-0022-843-H01C-AU21
GTGTGTATTATGAAGCCCAATAATTTATTAGTAAGTCAACCCATAGATAATTTAATTACT
TTACACAAAATTAAACAAATAAAAACATTAAAACACACGGAAATGAGCTTGGCCACCAAG
AAAAAAACAAAAACTTTTTTTATTTTGTTTTGGTTTTGGCTCTTCACCAAGAAAAAAAGA
TTCTATAGTACGGAATAAGTTTTCTTTTGGATGAAACTGAAATAATTTATCAAATAAACA
GAGAAGAAGAACCAAATTCAATGCTTCCTTGTCGCCGTCTTGTTCTGTCCTGCAAGAATC
AAGCTGCCAGTTTTCTCCGAAGGTTAGTCTTCTTATCCTTAAACCCCTTATAAACCCTAA
TTTTAAGTTGCTTAGGGGATTTTACTTTATCTGGGTTTTTTAAGTAGAACAATTGTTACT
CTATCTGCGAAAAAAGTCTCTCTTTGTTGGAATTTTGATGGTCTTGTGAATGTTTCTTGT
TTGGTTTATCTTCTAAAGTATCGAAATTCTTGATCTTAAAACATTATATTGAATCTTAGC
GTTGTCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11