Sequences from confirmation amplicon


Confirmation sequences for line 844D05 and hit At3g03870 are shown.
Line ID 844D05
Locus At3g03870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:995857
Primers used for PCR 37fm - 8474, show primer details
   
Sequence Name 28-K111392-0022-844-D05C-37fm
GenBank Accession LN498903 [GenBank]
Sequencing primer gene specific primer, 37fm: 5'-AACACCATCTCGGAAATAAAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K111392-0022-844-D05C-37fm
TATGTAAACAATTGTTAGTATATATTCGCCTCAAGACGAAACTACTCACATAAAACCAAT
AATTTTTCATTAAATTATTTAACTGAGAACATTCAAAGATATATCATAAAGAAAAATAAA
ATAAATTCATTTAACTAATTAAGAACATTACCTTATTATCATCGGAGTAAATCTGGAACC
GAAGCATCTGGTTCTTGTTCTTACAGATTTTGATATTGTTGTGTCCGAGGAAGAAGACAC
ATACGGTAGCAGCAGCAGCTGCAACCCCAAAAGAACAGATTGCTCCAATCCCATTGAGAC
CAGCCTTCCACAGATTCAATCTCTCACCACCACTACCATCTTCATCTTCTTCCTCCAAAA
CAACTTCTTTCTTGTCTCGCAAGTCATCCCCTCCTTCTAAATCAGAGGGTTTCGAAGCAG
CATCATCGATCTGAGGCAGAGGACTCGTGATCCTCGCTGGTGGGTCAATTTTCATGTCGG
CAAATTCAGTGTCCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 12-K111391-0022-844-D05C-8409
GenBank Accession LN491637 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K111391-0022-844-D05C-8409
TTCATTGAAGGACACTGAATTTGCCGACATGAAAATTGACCCACCAGCGAGGATCACGAG
TCCTCTGCCTCAGATCGATGATGCTGCTTCGAAACCCTCTGATTTAGAAGGAGGGGATGA
CTTGCGAGACAAGAAAGAAGTTGTTTTGGAGGAAGAAGATGAAGATGGTAGTGGTGGTGA
GAGATTGAATCTGTGGAAGGCTGGTCTCAATGGGATTGGAGCAATCTGTTCTTTTGGGGT
TGCAGCTGCTGCTGCTACCGTATGTGTCTTCTTCCTCGGACACAACAATATCAAAATCTG
TAAGAACAAGAACCAGATGCTTCGGTTCCAGATTTACTCCGATGATAATAAGGTAATGTT
CTTAATTAGTTAAATGAATTTATTTTATTTTTCTTTATGATATATCTTTGAATGTTCTCA
GTTAAATAATTTAATGAAAAATTATTGGTTTTATGTGAGTAGTTTCGTCTTGAGGCGAAT
ATATACTAACAATTGTTTTACATATTTAATCAGAAAACGATAAATGTAAAAAGTTTTTGT
GTTTTATTTCCGAGATGGTGT

Last Updated on 06.12.2018 16:11