Sequences from confirmation amplicon


Confirmation sequences for line 844D10 and hit At5g12130 are shown.
Line ID 844D10
Locus At5g12130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3920228
Primers used for PCR BF02 - 8474, show primer details
   
Sequence Name 03-K028743-022-844-D10C-BF02
GenBank Accession LN487350 [GenBank]
Sequencing primer gene specific primer, BF02: 5'-TCTGAGTCATGCTAATGTGCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028743-022-844-D10C-BF02
TCTCTCTCATCTTCCTCCTATCGCGTGTTCAGAGGTATTGATCAGGAAGATGAAGGTGAA
TGCTTCTTCTCCAATATTTTCTTCTGTTTTTTTTTTTCCGGAATTGTCGGTGTCAGTCTT
ACTTTTGCTAGCAAGACACAATTGTATATAAAGGAAGAAGCTTTATCTTGGGACAATAAT
TTGTTTATGGCATGTTAGTACAAAAAAGGAGAGCAAAGAACTTCTACCACACAAAAACGA
TGAGAATGCAACCACTTCACGCTCTTCTTCATCAGTAAATAGGGGAGGCCTGAAAAATTA
TCAACAAGAAAAAACTTACAAAACTTCCTTCAAGACTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCCCACCGTTATTATAAAATGAA
AGTACTTTTTGATAAAACGACAAATTACAATCCGCCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAGG
   
Sequence Name 03-K028743-022-844-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028743-022-844-D10C-8409
AAAAAATTTTTTTTTTTTGGAAATTTTTTTT

Last Updated on 06.12.2018 16:11