Sequences from confirmation amplicon


Confirmation sequences for line 844E05 and hit At5g42430 are shown.
Line ID 844E05
Locus At5g42430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16971540
Primers used for PCR DA47 - 8474, show primer details
   
Sequence Name 08-K107790-0022-844-E05C-DA47
GenBank Accession LN489761 [GenBank]
Sequencing primer gene specific primer, DA47: 5'-CCATTTCAAGAGTTCACGGTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107790-0022-844-E05C-DA47
TAACATTTTTCCCAATGAGAAGTGATACTCACAGGCCAAATAGCTAGAATGTAATAAATG
TTATGAAGAAAACTTCAGTCATGGTTGTGATTTGAATAAAACAAAAATGTACTGCAATAT
TATGAAGAAAACTTGCAAGGGAAGCATCAGTAAATTAACCATCATCAAAAAGACCCAATG
CATTGAATTTGCTAGCACTTGTTCCAGGTGAGCACCGAGCTTCACTTGATGATCATCTCT
AGGTGGGTTAAAGCTTCTGTTCTGATGGGCTTATTGATGTTGCATCATATTCATATGTTT
TCTTCATATGTTCTACAAAGGCGTAAACATTAAGATCCTCTACATGGTCTACAAAGGCGT
GAACACTACAACAGCTTTGGTATTCTCCAAAACCTTGGATTTCAACACTTAGGAGAGTGT
TCCTTTCGAGATTAAAGTAGAAGACATAAAACGGCTTACATACCTTTTTCTTCGCCTTAA
CGACAATCTATTTGATATATTCCACACAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 12-K107791-0022-844-E05C-8409
GenBank Accession LN491609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107791-0022-844-E05C-8409
GCCATTTACATTGAATATATCCTGTGTGGAATATATCAAATAGATTGTCGTTAAGGCGAA
GAAAAAGGTATGTAAGCCGTTTTATGTCTTCTACTTTAATCTCGAAAGGAACACTCTCCT
AAGTGTTGAAATCCAAGGTTTTGGAGAATACCAAAGCTGTTGTAGTGTTCACGCCTTTGT
AGACCATGTAGAGGATCTTAATGTTTACGCCTTTGTAGAACATATGAAGAAAACATATGA
ATATGATGCAACATCAATAAGCCCATCAGAACAGAAGCTTTAACCCACCTAGAGATGATC
ATCAAGTGAAGCTCGGTGCTCACCTGGAACAAGTGCTAGCAAATTCAATGCATTGGGTCT
TTTTGATGATGGTTAATTTACTGATGCTTCCCTTGCAAGTTTTCTTCATAATATTGCAGT
ACATTTTTGTTTTATTCAAATCACAACCATGACTGAAGTTTTCTTCATAACATTTATTAC
ATTCTAGCTATTTGGCCTGTGAGTATCACTTCTCATTTGGGAAAAATGTTATTTAATACC
TGAACTTTCAAAAAATGGCCAAATTAACCGTGAACTCTTGAAATGG

Last Updated on 06.12.2018 16:11