Sequences from confirmation amplicon


Confirmation sequences for line 844H12 and hit At1g05710 are shown.
Line ID 844H12
Locus At1g05710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1716741
Primers used for PCR 43ny - 8474, show primer details
   
Sequence Name 70-K114716-0022-844-H12C-43ny
GenBank Accession LN512437 [GenBank]
Sequencing primer gene specific primer, 43ny: 5'-GGGCATATTACACAAGTCCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114716-0022-844-H12C-43ny
ATCATTGCCAACCATGAGCTGTTCTAACCAACATCATGTGCTCTCTGTCTATATATGTTA
CAGAGGCATCATCAGAAAAATAACAATTGGTCATTCTTTTTTAGGCTTAACACAAAGATC
GTAGTAGGTGATTAGTCGAATTGGTCATCTAAAGGGTGATTGAGATGTGACACTGAAAGC
TGGTGATAGAGGAGTCTTCACGGGCGCCCATATATCAGCGCCGTTGCTTTGAGCAACTCC
AACTGTATTCTCCATTGGAACAAGACATAATCCTCTGTTTCTCAGGCTGTACTGCTCCAG
CTCCTCCTGGTTACAAAAGACGGGAAAATCAGTCCGTGCAAGGTATTCAAGTTCCAAGTG
AAGATCTGTCAGATGCAGAGTAGAACTAGTGAACTAAGTATAAATGAAGTTGAGATTCAA
GATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 22-K114715-0022-844-H12C-8409
GenBank Accession LN496223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114715-0022-844-H12C-8409
ATTTACATTGAATATGATCTTGAATCTCAACTTCATTTATACTTAGTTCACTAGTTCTAC
TCTGCATCTGACAGATCTTCACTTGGAACTTGAATACCTTGCACGGACTGATTTTCCCGT
CTTTTGTAACCAGGAGGAGCTGGAGCAGTACAGCCTGAGAAACAGAGGATTATGTCTTGT
TCCAATGGAGAATACAGTTGGAGTTGCTCAAAGCAACGGCGCTGATATATGGGCGCCCGT
GAAGACTCCTCTATCACCAGCTTTCAGTGTCACATCTCAATCACCCTTTAGATGACCAAT
TCGACTAATCACCTACTACGATCTTTGTGTTAAGCCTAAAAAAGAATGACCAATTGTTAT
TTTTCTGATGATGCCTCTGTAACATATATAGACAGAGAGCACATGATGTTGGTTTAGAAC
AGCTCATGGTTGGCAATGATTGTTATTATTATTCGACTGCTTAATGCATCCCCTTACTTT
GGACTTGTGTAATATGCC

Last Updated on 06.12.2018 16:11