Sequences from confirmation amplicon


Confirmation sequences for line 845C10 and hit At3g25760 are shown.
Line ID 845C10
Locus At3g25760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9404362
Primers used for PCR CS27 - 8474, show primer details
   
Sequence Name 76-K031321-022-845-C10C-8409
GenBank Accession LN513324 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K031321-022-845-C10C-8409
TTACAATTGAATAATCCTGCCAAAACAAAAAAATGAAATACTTACAAAACATTTTCTAAA
TACATTATCTTTATATTAAAATCAGTTTTTCATTTCATAATTTTTCTAACTCCATTAAAT
TTATATGATTCCAGAGCCTTAAAACCAAAAATTTGCAAACTTGATAATTTAAACATAATA
TCAGATCTCAAAATCAAGAACGTATAATTAATTCACAAATTGAGTTTTTAATTTTTCAGG
CAAAGTTCAAGAACTGAGCGTGTACGAAATCAATGATTTAGATCGACACAGCCCAAAAAT
TCTTAAAAACGCATTCAGCTTTAGGTTTGGTCTCGGAGATCTCGTCCCATTCACAAACAA
ACTCTACACCGGCGATCTCAAGAAACGCGTGGGAATCACGGCTGGTCTCTGCGTTGTGAT
CGAACACGTCCCTGAGAAGAACGGTGAATAGATT
   
Sequence Name 77-K031321-022-845-C10C-CS27
GenBank Accession LN513481 [GenBank]
Sequencing primer gene specific primer, CS27: 5'-GAATCTATCACCGTTCTTCTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K031321-022-845-C10C-CS27
TTCCACGCGTTTCTTGAGATCGCCGGTGTAGAGTTTGTTTGTGAATGGGACGAGATCTCC
GAGACCAAACCTAAAGCTGAATGCGTTTTTAAGAATTTTTGGGCTGTGTCGATCTAAATC
ATTGATTTCGTACACGCTCAGTTCTTGAACTTTGCCTGAAAAATTAAAAACTCAATTTGT
GAATTAATTATACGTTCTTGATTTTGAGATCTGATATTATGTTTAAATTATCAAGTTTGC
AAATTTTTGGTTTTAAGGCTCTGGAATCATATAAATTTAATGGAGTTAGAAAAATTATGA
AATGAAAAACTGATTTTAATATAAAGATAATGTATTTAGAAAATGTTTTGTAAGTATTTC
ATTTTTTTGTTTTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11