Sequences from confirmation amplicon


Confirmation sequences for line 846D01 and hit At1g33120 are shown.
Line ID 846D01
Locus At1g33120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12011649
Primers used for PCR KD50 - 8474, show primer details
   
Sequence Name 93-K034071-022-846-D01C-KD50
GenBank Accession LN515225 [GenBank]
Sequencing primer gene specific primer, KD50: 5'-AATCGCTTATGCTTTCCTTCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K034071-022-846-D01C-KD50
TTAGCTCAGAATATTCCTAGTTGATATTGTACAAGACTATGAGAAACACATTTTAACCAA
TAAAATATTGGTTGACCAGAGCGCATTAAATCAGTTATAGATGAATCAGTTCGATTTCAC
TATGCAAAATGTGATCCCAAACTCAAACATCAACCAAAACAAAAGAAATCTACGTCTCCT
ATACTACACAACGGCTCTATCTTTTCGACCCAAGATTAATTTCAAGTTGTTGTGGATATA
GTAGACTATGAGAGAATTGTGATATCTTCAGATCATTAAAACAAAATTGTGATATCTACA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 66-K034071-022-846-D01C-8409
GenBank Accession LN511539 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K034071-022-846-D01C-8409
GCCATGTAGATATCACAATTTNTGTTTTAATGATCTGAAGATATCACAATTCTCTCATAG
TCTACTATATCCACAACAACTTGAAATTAATCTTGGGTCGAAAAGATAGAGCCGTTGTGT
AGTATAGGAGACGTAGATTTCTTTTGTTTTGGTTGATGTTTGAGTTTGGGATCACATTTT
GCATAGTGAAATCGAACTGATTCATCTATAACTGATTTAATGCGCTCTGGTCAACCAATA
TTTTATTGGTTAAAATGTGTTTCTCATAGTCTTGTACAATATCAACTAGGAATATTCTGA
GCTAACTTGGCCGAGTTTGCATATTGTTGTATGAAGGAAAGCATAAGCGA
Primers used for PCR 86xm - 8474, show primer details
   
Sequence Name 41-K119432-0022-846-D01C-86xm
GenBank Accession LN504274 [GenBank]
Sequencing primer gene specific primer, 86xm: 5'-TCGACCTAAACTTGATAGACCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119432-0022-846-D01C-86xm
ATTTACTCAATTAATCCTAAACTCCTCTATTTGATCTGAACCCGATCAACCCGAATACAT
AAATATCTAATCTTATCCATCTGAAAGGATACTAAACTCTCTAATTCGAATTACCCGAAT
CCAGAAAAAGCCGAATAGCATGCATTCTATTGGTACTTGATTATATTAATTGGCATATAT
ATGACCTGTTATTGCCAATAATATATTTTGGGTGTTTCCTGGGCTTCAGTTTTATTGGGC
TAGGAATGGGTGTACTTTTAAACCCACTTCTGATTAGTCTCTTAGTAAAACCCACTTTTA
AACCCACTTCTGACCCACTTCTGACGCCGTCATTTTTCTTGAGAAGGAGAGAGAGCACCA
CTTCCGTCATTCCAGCCCAATATTTTTAACGGGCCATGGGCTTATTGTAAATAATGTATT
TTTCTCATTTTTGGTTTTCTTTATGGGCACGATTTTAGTGGGCTGAATTTTGATAAAGGC
CCACATATGAAAGATATTTAGGGTTTTTTCTCTTGATACCTTTTAAAACGCCGTCGAGAA
ATCTCCCTCTAGGGTTTCTTTGATCAAACCAGAGAGCCGTCAGGGAGAGCAAAACAAAGA
TCGGAGAAGATGAAGACGATTCTTTCTTCCGAAACGATGGACATCCCCGACAGTGTTACC
ATCAAGGTTCACGCTAAAGTGATCGAAGTCGAAGGACCTCGCGGGAAGCTTGTTCGCGAT
TTCAAGCATCTCAACCTCGATTTCCAGCTGATCAAGGATCCAGAGACTGGAAAGAAGAAG
CTTAAGATCGATTCGTGGTTTGGAACACGCAAAACCAGCGCCTCCATCAGAACCGCTCTT
AGCCACGTCGATAACTTGATCTCCGGTGTTACCAGAGGTTTTCGTTACAAGATGAGGTTC
GTGTACGCCCATTTTCCCATCAACGCCTCCATCGGCGGTGACGGAAAGTCTATCGAGATC
CGT
   
Sequence Name 01-K119431-0022-846-D01C-8409
GenBank Accession LN486805 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119431-0022-846-D01C-8409
ATTGAATATATCCTGGTTGATCAGAGCGCACGACCTTGAAACATCTCGATATCGTTACCG
TCAAGAACAATCTCATCCTTCACCTTCTCAGATCGAACAATGGTTACACCATCCAACATC
TCTACCTTCCTCACCTATTATTCCAATAAACACATTTCTATGTAAGTTCAGTGCTCGCAA
ACTCAGAAATCAATCATCATCGTCCAATCATAAAGTTGAATCTAGTGTCAGTCTATTTCA
TTCCGAGATCTACACGAATCTATGAGAATCAAATCAGAGATGAAGAAGATAAAACAAACC
TTCTTCTCGCCAAGGAAGTTACGGATCTCGATAGACTTTCCGTCACCGCCGATGGAGGCG
TTGATGGGAAAATGGGCGTACACGAACCTCATCTTGTAACGGAAACCTCTGGTAACACCG
GAGATCAAGTTATCGACGTGGCTAAGAGCGGTTCTGATGGAGGCGCTGGTTTTGCGTGTT
CCAAACCACGAATCGATCTTAAGCTTCTTCTTTCCAGTCTCTGGATCCTTGATCAGCTGG
AAATCGAGGTTGAGATGCTTGAAATCGCGAACAAGCTTCCCGCGAGGTCCTTCGACTTCG
ATCACTTTAGCGTGAACCTTGATGGTAACACTGTCGGGGATGTCCATCGTTTCGGAAGAA
AGAATCGTCTTCATCTTCTCCGATCTTTGTTTTGCTCTCCCTGACGGCTCTCTGGTTTGA
TCAAAGAAACCCTAGAGGGAGATTTCTCGACGGCGTTTTAAAAGGTATCAAGAGAAAAAA
CCCTAAATATCTTTCATATGTGGGCCTTTATCAAAATTCAGCCCACTAAAATCGTGCCCA
TAAAGAAAACCAAAAATGAGAAAAATACATTATTTACAATAAGCCCATGGCCCCGTAAAA
ATATTGGGCTGGAATGACGGAAGTGGTGCTCTCTC

Last Updated on 06.12.2018 16:11