Sequences from confirmation amplicon


Confirmation sequences for line 846H12 and hit At2g46140 are shown.
Line ID 846H12
Locus At2g46140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18959492
Primers used for PCR 36sf - 8474, show primer details
   
Sequence Name 33-K116491-0022-846-H12C-36sf
GenBank Accession LN501143 [GenBank]
Sequencing primer gene specific primer, 36sf: 5'-CCTCAAAATACAAAAGCTGAGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116491-0022-846-H12C-36sf
TATTAGTAATGTCATATTATGTATACAGTAGACTTGTGTAAATATATACATTAGGATTGA
TATAAGATGCTAGATATCATCAATGCACATGGATAATGAAATTAGTATATGTATGTATAT
ATAATGCGGCACAATGCGATAAAAATACAATTAGTGAGAAAATGAGATCTAGCCATTTCG
GTGATGAGACCAGACAAATTACCAACGAAATCCATACACATATATATTAAATACTGTTAT
CAATTAAAAATAGTCAAACATTAGATCGGATATACCTAATTGATACACATTTCTATTCAT
TAGTACAAAGATCGGATACACTTAACGAAAAATATCACATATATATTTTTCATATTCTCA
AAATTTAGATGATCAAAATATGTGTCACAAATTCTATTTATCTTAGGCTAGATCCGGAAT
ACGGATACTATCATGACGGCCGCCGAATGGAGCCACCGAATATGAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 01-K116490-0022-846-H12C-8409
GenBank Accession LN486732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116490-0022-846-H12C-8409
TTCTATTCGGTGGCTCCATTCGGCGGCCGTCATGATAGTATCCGTATTCCGGATCTAGCC
TAAGATAAATAGAATTTGTGACACATATTTTGATCATCTAAATTTTGAGAATATGAAAAA
TATATATGTGATATTTTTCGTTAAGTGTATCCGATCTTTGTACTAATGAATAGAAATGTG
TATCAATTAGGTATATCCGATCTAATGTTTGACTATTTTTAATTGATAACAGTATTTAAT
ATATATGTGTATGGATTTCGTTGGTAATTTGTCTGGTCTCATCACCGAAATGGCTAGATC
TCATTTTCTCACTAATTGTATTTTTATCGCATTGTGCCGCATTATATATACATACATATA
CTAATTTCATTATCCATGTGCATTGATGATATCTAGCATCTTATATCAATCCTAATGTAT
ATATTTACACAAGTCTACTGTATACATAATATGACATTACTAATAACACGTGTTATCAAG
GAAACAAAGGCACAAAGCTATATAGATGGTCTCAGCTTTTGATTTTTGAGG

Last Updated on 06.12.2018 16:11