Sequences from confirmation amplicon


Confirmation sequences for line 847E06 and hit At3g07200 are shown.
Line ID 847E06
Locus At3g07200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2291086
Primers used for PCR 22hg - 8474, show primer details
   
Sequence Name 48-K112776-0022-847-E06C-22hg
GenBank Accession LN506545 [GenBank]
Sequencing primer gene specific primer, 22hg: 5'-CACATTGATCCTAGGAGGAGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112776-0022-847-E06C-22hg
TGATCTCCCTGGTGTTCGTTTTGAGTCACACCATCCCTCCTTCGAAGTCTCATACTAGTC
CTCACCACCCGAGAAGCCATCTTTCAACAGAGTTAAGCTAACCTTACAGTCACAAAGTCA
CTACTGAATTAGAGTACTCTCAAGTTTAGGTCATCTTCGGAAAAAAAGAAGGACAAAGAT
TAACTTCTCAACAAAAAAATCAAAGATTTTGTCACATTTCAGAAGACATCCCCATGAATT
ACAAGAACTCAGGCAAAAAAATTCAAGATTTTTGTCAAAATTAGCCTAACGGAACTAAAA
ATAATAATAATTCAAACTTGGAAAAATCCAGTAAGGCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 25-K112774-0022-847-E06C-8409
GenBank Accession LN497521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112774-0022-847-E06C-8409
CATTTCATTGAATATATCCTGCCTTACTGGATTTTTCCAGTTTGAATTATTATTATTTTT
AGTTCCGTTAGGCTAATTTTGACAAAAATCTTGAATTTTTTTGCCTGAGTTCTTGTAATT
CATGGGGATGTCTTCTGAAATGTGACAAAATCTTTGATTTTTTTGTTGAGAAGTTAATCT
TTGTCCTTCTTTTTTTCCGAAGATGACCTAAACTTGAGAGTACTCTAATTCAGTAGTGAC
TTTGTGACTGTAAGGTTAGCTTAACTCTGTTGAAAGATGGCTTCTCGGGTGGTGAGGACT
AGTATGAGACTTCGAAGGAGGGATGGTGTGACTCAAAACGAACACCAGGGAGATCAACAA
GTGGCTGACCAAGCTCCAACGGTGCCTCAGATTGTGGCTACTCCTCCTAGATTCAATGTG

Last Updated on 06.12.2018 16:11