Sequences from confirmation amplicon


Confirmation sequences for line 847E10 and hit At1g62680 are shown.
Line ID 847E10
Locus At1g62680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23209431
Primers used for PCR 27vi - 8474, show primer details
   
Sequence Name 82-K117751-0022-847-E10C-27vi
GenBank Accession LN514235 [GenBank]
Sequencing primer gene specific primer, 27vi: 5'-ACTTCGTTGTAGGAGGATTCTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K117751-0022-847-E10C-27vi
ATCGATTGCGATGACGGCGAAGAGATTTCTTCATCGCAATCTTCTCGAGAATGGTAAACC
CAGAACTGCTTCTTCTCCTTCGTTTAGCCATTGCAGTTCCTGTCGTTGCTGGGTACGAGC
TTCTTCAAGCGTCAGTGGTGGTGATTTGCGAGAGAGATTAAGCAAAACTCGGCTTCGTGA
TATAAAGCTAAACGATGCGATTGATCTATTCAGTGACATGGTCAAGTCTCGTCCCTTTCC
TTCCATTGTCGATTTCAATAGATTGCTGAGTGCCATTGTTAAATTGAAGAAGTATGATGT
TGTTATCTCTTTGGGGAAGAAGATGGAAGTTTTGGGGATTCGAAATGATCTTTATACATT
TAACATTGTGATTAATTGTTTCTGTTGCTGTTTTCAAGTCTCTCTAGCTTTATCTATTCT
TGGCAAGATGTTGAAACTTGGTTATGAGCCAGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 57-K117750-0022-847-E10C-8409
GenBank Accession LN509533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117750-0022-847-E10C-8409
ATTTACATTGAATATATCTGGCTCATAACCAGTTTCAACATCTTGCCAAGAATAGATAAA
GCTAGAGAGACTTGAAAACAGCAACAGAAACAATTAATCACAATGTTAAATGTATAAAGA
TCATTTCGAATCCCCAAAACTTCCATCTTCTTCCCCAAAGAGATAACAACATCATACTTC
TTCAATTTAACAATGGCACTCAGCAATCTATTGAAATCGACAATGGAAGGAAAGGGACGA
GACTTGACCATGTCACTGAATAGATCAATCGCATCGTTTAGCTTTATATCACGAAGCCGA
GTTTTGCTTAATCTCTCTCGCAAATCACCACCACTGACGCTTGAAGAAGCTCGTACCCAG
CAACGACAGGAACTGCAATGGCTAAACGAAGGAGAAGAAGCAGTTCTGGGTTTACCATTC
TCGAGAAGATTGCGATGAAGAAATCTCTTCGCCGTCATCGCAATCGATCTCTGCATTTCG
ATGTTCGATTTTTGTCTCCGATGAGAATCCCTCCAACAAACGAA

Last Updated on 06.12.2018 16:11