Sequences from confirmation amplicon


Confirmation sequences for line 848G03 and hit At1g22300 are shown.
Line ID 848G03
Locus At1g22300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7879266
Primers used for PCR AQ04 - 8474, show primer details
   
Sequence Name 61-K106210-0022-848-G03C-AQ04
GenBank Accession LN510528 [GenBank]
Sequencing primer gene specific primer, AQ04: 5'-AGAGACAATCTCACCTTGTGGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K106210-0022-848-G03C-AQ04
GAGAGAGTATCTATTTTCTTTTGTTTCCCTATCTATGATCAATGGTTTCCTTAGAATCAA
ATGAGCTGTTAAGAGCGTTACCTTGGAGTAAAAATGTTGATGTTTGGGAACTCGTAACCA
TTAACCATCTGTCTATATTTGAATTAAACCGTCCTTTATGCATGTATTGCTATGTAGATC
TTTCATTTTAGTTTTGAAAGCCTCAACAAAAAACATACCAATCTACCATCTCATAGTTAT
TGGATTCTAGCTAATCTTGTAAAAGTTATATGTTTGTAGCAGAAAACATTACTAGTTTCT
TGTTTCTCTTCCAAAGCTTTTTCACTCACCTTTTTTTTTTCCCCTCTAAAATAAAAAAAC
CAAAGGTGCTAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGA
TCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAGAGTAATTACCATTTTTTTTT
CATTTCAAAAATGTAAATTTCC
   
Sequence Name 77-K106211-0022-848-G03C-8409
GenBank Accession LN513555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K106211-0022-848-G03C-8409
ATTTACAATTGAATATATCCTCAGCACCTTTGGTTCTCTCATCTAAGAGGGGAAAAAAAA
AAGGTGAGTGAAAAAGCTTTGGAAAAAAAACAAGAAACTAGTAATGTTTTCTGCTACAAA
CATATAACTTTTACAAAATTAGCTAGAATCCAATAACTATGAAATGGTAAATTGGTATGT
TTTTTGTTGAGGCTTTCAAAACTAAAATGAAAGATCTACATAGCAATACATGCATAAAGG
ACGGTTTAATTCAAATATAGACAAATGGTTAATGGTTACAAGTTCCCAAACATCAACATT
TTTACTCCAAGGTAACGCTCTTAACAGCTCATTTGATTCTAAGGAAACCATTTGATCATG
AGATAGGGAAACAAAAGAAAATAAAAATCCCTCCTTCCTCATTAAGGTCTGAAGTCCACA
AGGTGAATTTGTCT

Last Updated on 06.12.2018 16:11