Sequences from confirmation amplicon


Confirmation sequences for line 849D05 and hit At4g26760 are shown.
Line ID 849D05
Locus At4g26760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13480179
Primers used for PCR ME06 - 8474, show primer details
   
Sequence Name 83-K027351-022-849-D05C-8409
GenBank Accession LN514249 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K027351-022-849-D05C-8409
AATTGAATATANTCCNNTGAATCTATTCAAGNTGGGTTCATGGCTTCTGCATAATCATGG
GTTTTATGCAGCTTCAAGAACTAGCTACTCAGCTAACTGATCTATGGAATCTGATGGATA
CTTCTGATGAGGAAAGGGAGCTTTTTGACCATGTTACCTCTAACATTTCAGCTTCAGTTC
ATGAAGTGACTGCCTCTGGTGCTCTCGCACTTGATCTTATCGAACAGGTATCGTAAATTT
GTAATGTATCAATCAATGTGTATGATAAGTACTGCTTTTAAAAATCTTCTGTGAACTCTT
GAAGGCTGAGGTGGAAGTGGATAGGCTTGATCAACTGAAATCTAGCAGAATGAAGGAAAT
TGCATTCAAAAAGCAATCAGAGCTTGAGGAGATATATGCTCGTGCTCACATAGAAATAAA
ACCAGAGGTTGTTCGTGAGAGGATCATGTCGTTGATTGATGCTGGGAA
   
Sequence Name 84-K027351-022-849-D05C-ME06
GenBank Accession LN514366 [GenBank]
Sequencing primer gene specific primer, ME06: 5'-CTCAGTAGGTTCAGTATTCCCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K027351-022-849-D05C-ME06
GACACCTCTGGTTTATTTCTATGTGAGCACGAGCATATATCTCCTCAAGCTCTGATTGCT
TTTTGAATGCAATTTCCTTCATTCTGCTAGATTTCAGTTGATCAAGCCTATCCACTTCCA
CCTCAGCCTTCAAGAGTTCACAGAAGATTTTTAAAAGCAGTACTTATCATACACATTGAT
TGATACATTACAAATTTACGATACCTGTTCGATAAGATCAAGTGCGAGAGCACCAGAGGC
AGTCACTTCATGAACTGAAGCTGAAATGTTAGAGGTAACATGGTCAAAAAGCTCCCTTTC
CTCATCAGAAGTATCCATCAGATTCCATAGATCAGTTAGCTGAGTAGCTAGTTCTTGAAG
CTGCATAAAACCATGATTATGCAGAAGCATGAACCACTTGAATAGATTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTATTTCGACGTGTCT
A

Last Updated on 06.12.2018 16:11