Sequences from confirmation amplicon


Confirmation sequences for line 849G11 and hit At3g57780 are shown.
Line ID 849G11
Locus At3g57780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21400171
Primers used for PCR NR87 - 8474, show primer details
   
Sequence Name 39-K102592-0022-849-G11C-8409
GenBank Accession LN503312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102592-0022-849-G11C-8409
GCCATTTAAATTGAATATATCGTTATTAAGCTCTTCTAGGACAGCTCCAGGGACATCATC
AGGACAAAACTCATCAGGTGTAAAGTTGCAGAGTATTCTTTTGATCAACGCAAGACTTAT
CGATGGACATACCTATAAATCAACCAAGAATAAAGGCTCTGTGGTCAAGAAAGTTTAATC
AAGAACTTAAAGCGTGATAATGAAAGATAACAAAACCTCTTCTCTAGTGGATCGGTCCAT
AAGCATGTCTTTTGGAAGCATAAGTAGATCACTGAGTTCGTTTAGCAAACTGAAAGATTT
CTCACTTTCTATTGGATCATTTTCTTCAACTGAATCTCTAGTGTTTATGGAGAACATTTC
TGCAAGGCATCTAGACCAATTCCCAATCTACAAGCGAGAGATCACAACATTCAAAAACAT
TAGTACTTTCTCATGTTAAGTGAATATAACAATCTGATTATGTTTCTTAATGTTGTACCG
CGTTTTTAAGCTGTGCACCGGATCCAAAGCTTAAGTTTCCAGAAAGGATAGGCAGACTGG
   
Sequence Name 39-K102593-0022-849-G11C-NR87
GenBank Accession LN503313 [GenBank]
Sequencing primer gene specific primer, NR87: 5'-CTTGATTCCAAAGTTCTGCCTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102593-0022-849-G11C-NR87
CTTAAAACGCGGTACAACATTAAGAAACATAATCAGATTGTTATATTCACTTAACATGAG
AAAGTACTAATGTTTTTGAATGTTGTGATCTCTCGCTTGTAGATTGGGAATTGGTCTAGA
TGCCTTGCAGAAATGTTCTCCATAAACACTAGAGATTCAGTTGAAGAAAATGATCCAATA
GAAAGTGAGAAATCTTTCAGTTTGCTAAACGAACTCAGTGATCTACTTATGCTTCCAAAA
GACATGCTTATGGACCGATCCACTAGAGAAGAGGTTTTGTTATCTTTCATTATCACGCTT
TAAGTTCTTGATTAAACTTTCTTGACCACAGAGCCTTTATTCTTGGTTGATTTATAGGTA
TGTCCATCGATAAGTCTTGCGTTGATCAAAAGAATACTCTGCAACTTTACACCTGATGAG
TTTTGTCCTGATGATGTCCCTGGAGCTGTCCTAGAAGAGCTTAATAACGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATT

Last Updated on 06.12.2018 16:11