Sequences from confirmation amplicon


Confirmation sequences for line 849H01 and hit At4g27100 are shown.
Line ID 849H01
Locus At4g27100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13596779
Primers used for PCR GF07 - 8474, show primer details
   
Sequence Name 16-K035349-022-849-H01C-GF07
GenBank Accession LN493346 [GenBank]
Sequencing primer gene specific primer, GF07: 5'-CTGAACCATTTGAGTTTGTACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K035349-022-849-H01C-GF7
TGCCTATTACAAGTTTATGTAAGAGTTCAACATTAAATTTCCGTAATAGAAAGGCAAAAT
TTTACCTAAATTTATTTCCTGAAGTACAAGAGGATCATAGTTGGGTTGACCATTTTCAGT
AATTACAGGTGCGGTAGTAAATCTTCCACTTCCAATGACAGGAAACATTTGCTTACACTC
TTCCTTCCAAGAAGCATACTGCAATCTGAAAGAAGAGAATTAGCGGTAGGATTCACAAAA
CTGAATCGATGGTTTATGAATGAACTGAAAGAAACATGTAATTATGCCAAATGCGATACA
TATATATAGGATATGAGGACAAAATTCTACCTTCTGCGTTGTCGAATTTGCTCTCTTTCT
TCGAATGTGCTCATTGGGTCATAACAACCAAGTAGGAATTCTACATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 08-K035349-022-849-H01C-8409
GenBank Accession LN489643 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K035349-022-849-H01C-8409
CCATGTAGAATTCCTACTTGGTTGTTATGACCCAATGAGCACATTCGAAGAAAGAGAGCA
AATTCGACAACGCAGAAGGTAGAATTTTGTCCTCATATCCTATATATATGTATCGCATTT
GGCATAATTACATGTTTCTTTCAGTTCATTCATAAACCATCGATTCAGTTTTGTGAATCC
TACCGCTAATTCTCTTCTTTCAGATTGCAGTATGCTTCTTGGAAGGAAGAGTGTAAGCAA
ATGTTTCCTGTCATTGGAAGTGGAAGATTTACTACCGCACCTGTAATTACTGAAAATGGT
CAACCCAACTATGATCCTCTTGTACTTCAGGAAATAAATTTAGGTAAAATTTTGCCTTTC
TATTACGGAAATTTAATGTTGAACTCTTACATAAACTTGTAATAGGCATTTGAAGTTTTC
ATGTGGCTTTCTCAGGTACAAACTCAAATGGTTCAG

Last Updated on 06.12.2018 16:11