Sequences from confirmation amplicon


Confirmation sequences for line 850D04 and hit At1g78580 are shown.
Line ID 850D04
Locus At1g78580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29555746
Primers used for PCR 38ai - 8474, show primer details
   
Sequence Name 33-K106773-0022-850-D04C-8409
GenBank Accession LN501015 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K106773-0022-850-D04C-8409
ATCCGAAGCAATACATAATTCTGTTACAAGGCTTTCAAACAATGACAAGGTATTATTAGC
TTTTCTATCACTGCGGCATGATAAGTGTAAATGGTCAAAGTAATGAAATGGTCATACAGC
AGCTACACGAGTGAGCCTGCCTTGATCCTCAACTCCCTCAGGTGTTCCTTCAAGTCCAAG
AATACGAGTGCACGCACTCACAAAGTGCCTTGCATAGTCATATGTATGGAAGCTGTAAAG
TAAACAATGTGTCATGTTACTTTCCGAAAACAGAATAAAGATCCATCAGCTCATTACTAA
AATAACCAATGTAGTATTTTTACCATTGGATCCAAATTATACACAGTCAAAGTAAATTCT
TACACCACCTAAAAGAACTGAATCATTACCAACAATCAGATCCTAGATATATCAAGCTAC
ACATGGTTAACTATTTCACCTCTATATATGTACCATGGAGATAAATTATTCGATATTTAG
ACTAGTTCTCAACCAGACAAACCACTTAAGATTGGTTGAAACAGTAACACATAGATTTGA
ACCTGTCACATGGAGCTAATACCATGGAAGATCACTTGCAATACATAGAAGCTAGTAGAG
ATTTTTACCCAACTAAATCAGCAGCAAGAACTGAGCGAAGGAGCTCTGATCGTGATGGAA
GTGTCCTGTGTATCTCAGACGAAGGGAATGGTGTATGGAGAA
   
Sequence Name 37-K106774-0022-850-D04C-38ai
GenBank Accession LN502629 [GenBank]
Sequencing primer gene specific primer, 38ai: 5'-AAGTTGGATGGTTTCTCCATACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K106774-0022-850-D04C-38ai
CGATCAGAGCTCCTTCGCTCAGTTCTTGCTGCTGATTTAGTTGGGTAAAAATCTCTACTA
GCTTCTATGTATTGCAAGTGATCTTCCATGGTATTAGCTCCATGTGACAGGTTCAAATCT
ATGTGTTACTGTTTCAACCAATCTTAAGTGGTTTGTCTGGTTGAGAACTAGTCTAAATAT
CGAATAATTTATCTCCATGGTACATATATAGAGGTGAAATAGTTAACCATGTGTAGCTTG
ATATATCTAGGATCTGATTGTTGGTAATGATTCAGTTCTTTTAGGTGGTGTAAGAATTTA
CTTTGACTGTGTATAATTTGGATCCAATGGTAAAAATACTACATTGGTTATTTTAGTAAT
GAGCTGATGGATCTTTATTCTGTTTTCGGAAAGTAACATGACACATTGTTTACTTTACAG
CTTCCATACATATGACTATGCAAGGCACTTTGTGAGTGCGTGCACTCGTATTCTTGGACT
TGAAGGAACACCTGAGGGAGTTGAGGATCAAGGCAGGCTCACTCGTGTAGCTGCTGTATG
ACCATTTCATTACTTTGACCATTTACACTTATCATGCCGCAGTGATAGAAAAGCTAATAA
TACCTTGTCATTGTTTGAAAGCCTTGTAACAGAATTATGTATTTGCTTCGGATTTAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11